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HCYP2SF-62K MILLIPLEX® Human Cytokine/Chemokine Panel II Serum-Free. Available in China only. All other countries, please order HCYP2MAG-62K.

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.

HCYP2SF-62K

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Overview

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Description
Catalogue NumberHCYP2SF-62K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Cytokine/Chemokine Panel II Serum-Free. Available in China only. All other countries, please order HCYP2MAG-62K.
OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
• More flexible plate and plate washer options
• Improved performance with turbid serum/plasma samples
• Assay results equivalent to non-magnetic beads
• Automated magnetic washing avoids many problems associated with vacuum filtration washing

Therefore, the MILLIPLEX® MAP Human Cytokine / Chemokine Panel II enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

References:
PLoS One. 2016 Feb 26;11(2):e0149411. doi: 10.1371/journal.pone.0149411. eCollection 2016.
B Cell, Th17, and Neutrophil Related Cerebrospinal Fluid Cytokine/Chemokines Are Elevated in MOG Antibody Associated Demyelination.
Kothur K, Wienholt L, Tantsis EM, Earl J, Bandodkar S, Prelog K, Tea F, Ramanathan S, Brilot F, Dale RC
Background Information“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: 4 - 9
  • Inter-assay: 6 - 17
Multiplex Ordering RequirementsFor questions, contact Technical Service.
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.
Key Applications
  • Multiplexing
Application NotesThis is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.
This assay requires 25 µl cell culture supernatant per well.
This kit may be used for the analysis of all or any combination of the cytokines and chemokines in tissue/cell lysate and culture supernatant samples.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • 6Ckine
  • BCA-1
  • CTACK
  • ENA-78
  • Eotaxin-2
  • Eotaxin-3
  • I-309
  • IL-16
  • IL-20
  • IL-21
  • IL-23
  • IL-28A
  • IL-33
  • LIF
  • MCP-2
  • MCP-4
  • MIP-1d
  • SCF
  • SDF-1A+β
  • TARC
  • TPO
  • TRAIL
  • TSLP
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies and any of the other analytes in this panel.
Accuracy
  • 81% - 110%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual analytes.
Linearity of DilutionContact Technical Service for this value
Standard Curve Range
  • BCA-1, Tarc: 1 - 1,000 pg/mL
  • I-309 - 2 - 2,000 pg/mL
  • MCP-2, CTACK: 4.9 - 5,000 pg/mL
  • MCP-4, Eotaxin-2, TRAIL, SCF, TSLP, IL-28A, IL-16: 9.8 - 10,000 pg/mL
  • ENA-78, 6Ckine, LIF, IL-21, IL-33: 19.5 - 20,000 pg/mL
  • MIP-1δ, Eotaxin-3, IL-23, TPO, IL-20: 48.8 - 50,000 pg/mL
  • SDF-1α+β: 97.7 - 100,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HCYP2SF-62K 04065267238180

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