Millipore Sigma Vibrant Logo

HT17SF-14K-PX25 MILLIPLEX® Human TH17 Panel Premixed Serum-Free. Available in China Only. All other countries, please order HT17MG-14K-PX25.

Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.

HT17SF-14K-PX25
96-well plate  96 well plate
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Catalogue NumberHT17SF-14K-PX25
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® Human TH17 Panel Premixed Serum-Free. Available in China Only. All other countries, please order HT17MG-14K-PX25.
      OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. Each MILLIPLEX® panel and kit includes:
      • Quality controls (QCs) provided to qualify assay performance
      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
      • Detection antibody cocktails designed to yield consistent analyte profiles within panel
      Background InformationCD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.

      Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth.

      The MILLIPLEX® MAP Human TH17 Magnetic Bead Panell enables you to focus on the relevance and therapeutic potential of the modulation of CD4 T-helper cell cytokines in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Inter-assay: < 15
      • Intra-assay:< 10
      Multiplex Ordering RequirementsPremixed
      Panel TypeMAGNETIC Cytokines/Chemokines
      Quality LevelMQ200
      Applications
      Application Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight incubation assay requiring 25 µL of cell culture supernatant per well.
      • This kit may be used for the analysis of all of the Th17 panel analytes in tissue/cell lysate and culture supernatant
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • GM-CSF
      • IFN-γ
      • IL-1β
      • IL-2
      • IL-4
      • IL-5
      • IL-6
      • IL-9
      • IL-10
      • IL-12 (p70)
      • IL-13
      • IL-15
      • IL-17A
      • IL-17F
      • IL-17E/IL-25
      • IL-21
      • IL-22
      • IL-23
      • IL-27
      • IL-28A
      • IL-31
      • IL-33
      • MIP-3α/CCL20
      • TNF-α
      • TNFβ
      Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Accuracy
      • 79 - 107%
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities of individual analytes.
      Standard Curve Range
      • IL-17 & IL-4, 24 - 100,000 pg/mL
      • GM-CSF & IL-27, 61 - 250,000 pg/mL
      • IFNγ, 10 - 40,000 pg/mL
      • IL-10, 1 - 5000 pg/mL
      • MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33, 5 - 20,000 pg/mL
      • IL-13, 7 - 30,000 pg/mL
      • IL-17A & IL-2, 12 - 50,000 pg/mL
      • IL-22 & TNFβ, 37 - 150,000 pg/mL
      • IL-9, 9 - 35,000 pg/mL
      • IL-23, 336 - 1,500,000 pg/mL
      • IL-5, 6 - 25,000 pg/mL
      • IL-6 & TNFα, 3 - 10,000 pg/mL
      • IL-17E/IL-25, 488 - 2,000,000
      • IL-28a & IL-31, 49 - 200,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      HT17SF-14K-PX25 04065267238395

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      HT17MAG14PMX25BK MILLIPLEX MAP Human TH17 Magnetic Bead Panel Premixed - 25 Plex - Space Saver (Bulk) Packaging Show Pricing & Availability
      HTH17MAG-14K MILLIPLEX MAP Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay Show Pricing & Availability