48-683MAG MILLIPLEX MAP Bcl-2 Family Apoptosis Panel 2 Magnetic Bead 4-plex Kit - Cell Signaling Multiplex Assay

48-683MAG
96-well plate  96-well plate
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Bcl-xL, Mcl-1, BCL-2 (Total), NOXA/Mcl-1HMplexLuminex xMAP
      Description
      Catalogue Number48-683MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Bcl-2 Family Apoptosis Panel 2 Magnetic Bead 4-plex Kit - Cell Signaling Multiplex Assay
      OverviewApoptosis is an essential physiological process that plays a crucial role in normal development and homeostatic mechanisms of multicellular organisms. At least two major apoptotic pathways have been described in mammalian cells: the intrinsic pathway via Caspase-9 activation, in which death arises from mitochondrial dysfunction, and the extrinsic pathway via Caspase-8 activation, in which death is initiated from the activation of cell surface receptors. Bcl-2 family members play an important role in the intrinsic apoptotic pathway and includes over 17 members. Interactions of these Bcl-2 family members function as a ‘life/death switch’ that integrates diverse inter- and intracellular cues to determine whether or not the stress apoptosis pathway should be activated. For example, Bcl-2 and its closely related anti-apoptotic proteins, Bcl-xL and Mcl-1, can sequester their pro-apoptotic counterparts (BAD, BIM, NOXA and PUMA) and thereby inhibit the initiation steps of apoptosis.

      The MILLIPLEX® MAP 4-plex Bcl-2 Family Apoptosis Panel 4 Magnetic Bead Kit (Cat. No. 48-683MAG) is a premixed kit to be used for the simultaneous quantification of the following 4 analytes:

      • Bcl-xL
      • Mcl-1
      • Bcl-2
      • NOXA/Mcl-1
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-Assay: < 15
      • Inter-Assay: < 20
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 1 (provided).
      • The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • Bcl-xL
      • Mcl-1
      • BCL-2 (Total)
      • NOXA/Mcl-1
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications