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RLI1MAG-92K MILLIPLEX MAP Rat Liver Injury Panel - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), aspartate transaminase 1 (GOT1), α-glutathianone S-transferase (GSTα), sorbitol dehydrogenase (SDH), and 5'-Nucleotidase (5'-NT/CD73).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Toxicity Research Sub-Category: Toxicity
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RLI1MAG-92K
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Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ARG1 GOT1 GSTα SDH 5'-NT/CD73 RMplexLuminex xMAP
Description
Catalogue NumberRLI1MAG-92K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Rat Liver Injury Panel - Toxicity Multiplex Assay
OverviewThe MILLIPLEX® MAP Rat Liver Injury Magnetic Bead Panel (RLI1MAG-92K) contains all the components necessary to simultaneous quantify the following 5 analytes in serum and plasma samples:

• Liver-Type Arginase 1 (ARG1)*
• Aspartate transaminase 1 (GOT1)
• α-glutathione S-transferase (GSTα)*
• Sorbitol Dehydrogenase (SDH)*
• 5'-Nucleotidase/CD73 (5’-NT)

*ARG1, GSTα, and SDH are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.

EMD Millipore provides a valuable research assay to investigate multiple biomarkers of liver injury in rat serum and plasma samples using the Luminex xMAP® instrument platform. This kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum or plasma samples in duplicate.
Background InformationDrug toxicity is the leading cause of acute liver failure in the United States. Patients with liver damage generally display elevated amounts of specific liver proteins in serum; these proteins can serve as biomarkers of drug-related liver toxicity. Monitoring these biomarkers can greatly help clinicians avoid drug-induced liver failure. Performing laboratory tests to characterize the side effects of potential therapeutics is an essential part of drug development. The rat is a leading animal model for these pre-clinical toxicity studies.

The search for sensitive, organ-specific toxicity biomarkers is complemented by the development of novel assays to measure these critical analytes. Liver-Type Arginase 1 (ARG1), α-glutathione S-transferase (GSTα), and Sorbitol Dehydrogenase (SDH) are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. Aspartate transaminase 1 (GOT1) and 5'-Nucleotidase / CD73 (5’-NT) are traditional biomarkers recognized by both the Food and Drug Administration (FDA) and its European counterpart, the European Medicines Agency (EMA).
References
Product Information
Components
  • Rat Liver Injury Standard, 1 vial, lyophilized
  • Rat Liver Injury Quality Control 1, 1 vial, lyophilized
  • Rat Liver Injury Quality Control 2, 1 vial, lyophilized
  • Assay Buffer, 1 bottle, 30 mL
  • Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
  • Rat Liver Injury Detection Antibodies, 1 bottle, 3.2 mL
  • Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
  • Mixing Bottle, 1 bottle
  • Sample Plate, 96 well format, 1 plate
  • 2 Foil Plate Sealers
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15
  • Intra-assay: <10
Panel TypeMAGNETIC Toxicity
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), aspartate transaminase 1 (GOT1), α-glutathianone S-transferase (GSTα), sorbitol dehydrogenase (SDH), and 5'-Nucleotidase (5'-NT/CD73).
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay.
• This assay requires 25 µL of 1:25 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions).
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Rat
Analytes Available
  • ARG1
  • GOT1
  • GSTα
  • SDH
  • 5'-NT/CD73
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 77% - 92%
Physicochemical Information
Sensitivity
  • 0.021 - 4.038 ng/mL (MinDC+2SD).
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionContact technical support for this information.
Standard Curve Range
  • ARG1: 0.10 – 75.0 ng/mL
  • GOT1: 0.08 – 60.0 ng/mL
  • GSTα: 0.02 – 15.0 ng/mL
  • SDH: 0.62 – 450.0 ng/mL
  • 5'-NT/CD73: 0.04 – 30.0 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
RLI1MAG-92K 04055977130737

Documentation

Protocols

Title
Protocol- RLI1MAG-92K

MILLIPLEX MAP Rat Liver Injury Panel - Toxicity Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Development of a MILLIPLEX®MAP multiplexed assay panel for emerging biomarkers
Quality Control Range Sheet- RL192-104 and RL192-204
Quality Control Ranges- Milliplex Rat Liver Injury Magnetic Panel
White Paper: Further considerations of antibody validation and usage.

Posters

Title
Toxicology in drug discovery and development: Biomarkers and model organisms