48-660MAG MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

48-660MAG
96-well plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      ATF2 (Thr71), Erk (Thr185/Tyr187), HSP27 (Ser78), JNK (Thr183/Tyr185), c-Jun (Ser73), MEK1 (Ser222), MSK1 (Ser212), p38 (Thr180/Tyr182), p53 (Ser15), STAT1 (Tyr701)HMplexLuminex xMAP
      Description
      Catalogue Number48-660MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay
      OverviewCells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-Assay: 3% - 10%
      • Inter-Assay: 4% - 13%
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate.
      • This kit must be run using Assay Buffer 2 (provided).
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • ATF2 (Thr71)
      • Erk (Thr185/Tyr187)
      • HSP27 (Ser78)
      • JNK (Thr183/Tyr185)
      • c-Jun (Ser73)
      • MEK1 (Ser222)
      • MSK1 (Ser212)
      • p38 (Thr180/Tyr182)
      • p53 (Ser15)
      • STAT1 (Tyr701)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      10-Plex MAPK/SAPK Signaling Magnetic Bead Panel

      MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Mechanism of Siglec-8-mediated cell death in IL-5-activated eosinophils: role for reactive oxygen species-enhanced MEK/ERK activation.
      Kano, G; Almanan, M; Bochner, BS; Zimmermann, N
      The Journal of allergy and clinical immunology  132  437-45  2013

      Show Abstract
      23684072 23684072

      Brochure

      Title
      Custom Immunoassay Menu of Services
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
      The Power of Biomarker Analysis

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single

      Data Sheet

      Title
      Full Service Custom-built Assays

      Posters

      Title
      MILLIPLEX MAP: Exercise, Signaling, and Your Brain
      MILLIPLEX® MAP Cell Signaling Wall Chart (MM)