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HPLX1-115SP-PX80 MILLIPLEX® PLEXpedition 80-plex Screening Premixed Kit

•GIP and ENA-78 have low cross-reactivity with IL-23 (≤15%) and GRO (≤5%) recombinant proteins, respectively.
•IL-12 (p40) has moderate, but expected, cross-reactivity with the recombinant proteins for IL-12 (p70) and IL-23 which both contain the p40 subunit.
•MMP-3 may exhibit significant cross-reactivity with MMP-10 recombinant proteins due to high sequence homology, however, analysis demonstrated independent and differentiated measurement of MMP-3 and MMP-10 in biological samples.

HPLX1-115SP-PX80
96-well plate  96 well plate
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      Overview

      Replacement Information
      Description
      Catalogue NumberHPLX1-115SP-PX80
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX® PLEXpedition 80-plex Screening Premixed Kit
      OverviewThe MILLIPLEX® PLEXpedition 80-plex Screening Premixed Kit is a configurable multiplex immunoassay kit developed to simultaneously screen up to 80 targets including cytokines, chemokines, growth factors, matrix metalloproteinases (MMPs), and biomarkers of bone health, metabolism, and cardiovascular disease. The Luminex® 200™, the xMAP® INTELLIFLEX, and the FLEXMAP 3D® can analyze all 80 bead regions. As a discovery biomarker tool this panel is provided in a matrix-free format to enable analyte detection in multiple sample types. After screening with PLEXpedition to identify markers of interest, customers can transition to our Qualified MILLIPLEX® panels, each of which has an optimized serum matrix and lot-to-lot verification for reproducible quantification of protein concentration.

      MILLIPLEX® Screening Assays
      • Contain all reagents necessary to conveniently run the assay including premixed beads and premixed detection antibodies
      • Verify analyte specificity across the entire panel
      • Are sample agnostic for greater flexibility
      • Enable easy transition to Qualified Assays by utilizing same critical raw materials
      Background InformationThe MILLIPLEX® PLEXpedition Magnetic Bead Screening Panel is to be used for the simultaneous quantification of 80 analytes in your selected sample type.

      The Luminex® 200™, FLEXMAP 3D®, or xMAP® INTELLIFLEX is required to analyze the 80 analytes included in this kit: ACTH, BDNF, C-Peptide, EGF, Eotaxin, FABP4, FGF-2, FLT3 Ligand, Fractalkine, G-CSF, Ghrelin (Active), GIP, GLP-1 Total, Glucagon, GM-CSF, Granzyme A, Granzyme B, GROα, HGF, IFNα2, IFNγ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, Insulin, IP-10, I-TAC, Leptin, MCP-1, MCP-3, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-3α, MMP-1, MMP-2, MMP-7, MMP-9, OPG, OPN, PDGF-AA, PDGF-BB, Perforin, PlGF, PP, PYY, RANTES, sCD40L, SCF, sFAS, sFASL, TARC, TGFα, TNFα, TNFβ, TRAIL, Troponin I, VEGF-A.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-assay: <15%
      • Inter-assay: <20%
      • (<25% for PDGF-AA)
      Panel TypeMAGNETIC Immune Response
      Quality LevelMQ100
      Applications
      Application•GIP and ENA-78 have low cross-reactivity with IL-23 (≤15%) and GRO (≤5%) recombinant proteins, respectively.
      •IL-12 (p40) has moderate, but expected, cross-reactivity with the recombinant proteins for IL-12 (p70) and IL-23 which both contain the p40 subunit.
      •MMP-3 may exhibit significant cross-reactivity with MMP-10 recombinant proteins due to high sequence homology, however, analysis demonstrated independent and differentiated measurement of MMP-3 and MMP-10 in biological samples.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight assay.
      • Each assay requires 25 µL per well of neat or diluted sample.
      • Each kit includes premixed beads and premixed detection antibodies.
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • ACTH
      • BDNF
      • C-Peptide
      • EGF
      • Eotaxin
      • FABP4
      • FGF-2
      • Flt-3 ligand
      • Fractalkine
      • G-CSF
      • Ghrelin (Active)
      • GIP
      • GLP-1 (Total)
      • Glucagon
      • GM-CSF
      • Granzyme A
      • Granzyme B
      • GROα
      • HGF
      • IFNα2
      • IFNγ
      • IL-10
      • IL-12 (p40)
      • IL-13
      • IL-15
      • IL-17A
      • IL-17F
      • IL-18
      • IL-1Rα
      • IL-1α
      • IL-1β
      • IL-2
      • IL-21
      • IL-22
      • IL-23
      • IL-3
      • IL-33
      • IL-4
      • IL-5
      • IL-6
      • IL-7
      • IL-8
      • IL-9
      • Insulin
      • IP-10
      • I-TAC
      • Leptin
      • MCP-1
      • MCP-3
      • M-CSF
      • MDC (CCL22)
      • MIG
      • MIP-1α
      • MIP-1β
      • MIP-3α
      • MMP-1
      • MMP-2
      • MMP-7
      • MMP-9
      • OPG
      • OPN - Osteopontin
      • PDGF-AA
      • PDGF-BB
      • Perforin
      • PIGF-2
      • PP
      • PYY
      • RANTES
      • sCD40L
      • SCF
      • sFas
      • sFasL
      • TARC
      • TGFα
      • TNFα
      • TNFβ
      • TRAIL
      • Troponin I (TnI)
      • VEGF-A
      • IL-12 (p70)
      Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, with the following exceptions: (See Applications)
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities for individual analytes.
      Standard Curve Range
      • Refer to lot specific product inserts for analyte concentrations.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      HPLX1-115SP-PX80 04065270783219

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