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444282 MMP/TACE Substrate, FS-6 - Calbiochem

The widely used MMP substrate FS-1 is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities.

MMP/TACE Substrate, FS-6 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mca-KPLG~L-Dpa-AR-NH₂

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      Overview

      Replacement Information
      Description
      OverviewThe widely used MMP substrate FS-1 (Cat. No. 03-32-5032) is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities. Exhibits 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 when compared with FS-1 and ~2-fold higher kcat/Km towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10.
      Catalogue Number444282
      Brand Family Calbiochem®
      SynonymsMca-KPLG~L-Dpa-AR-NH₂
      References
      ReferencesNeumann, U., et al. 2004. Anal. Biochem. 328, 166.
      Product Information
      ATP CompetitiveN
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₅₅H₈₀N₁₆O₁₆
      Chemical formulaC₅₅H₈₀N₁₆O₁₆
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetBroad-spectrum MMP and TACE substrate
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableN
      Peptide SequenceMca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      444282 0

      Documentation

      MMP/TACE Substrate, FS-6 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      MMP/TACE Substrate, FS-6 - Calbiochem Certificates of Analysis

      TitleLot Number
      444282

      References

      Reference overview
      Neumann, U., et al. 2004. Anal. Biochem. 328, 166.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-September-2007 RFH
      SynonymsMca-KPLG~L-Dpa-AR-NH₂
      DescriptionThe widely used MMP substrate FS-1 (Cat. No. 03-32-5032), modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and sensitivity. Exhibits a 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 (Km (μM)= 27.5, 5.2, and 7.9, respectively) when compared with FS-1 and ~ 2-fold higher kcat/Km (778, 400 M-1 s-1) towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10. Excitation max: 324 nm; Emission max: 400 nm.
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditionsMMP: 100 mM Tris-HCL,100 mM Nacl, 10 mM CaCl2, 0-5% (w/v) BRIJ®35 detergent, 0.1% (w/v) PEG6000, pH7.5. TACE and ADAM10: (10 mM HEPES, pH 7.5).
      Chemical formulaC₅₅H₈₀N₁₆O₁₆
      Peptide SequenceMca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
      Purity≥97% by HPLC
      SolubilityH₂O (20 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesNeumann, U., et al. 2004. Anal. Biochem. 328, 166.