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71256 MagPrep® Bacterial Genomic DNA Kit

Efficient isolation of genomic DNA from Gram-positive and Gram-negative bacterial species

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

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      This product has been discontinued.





      The MagPrep® Bacterial Genomic DNA Kit is designed for purification of genomic DNA from both Gram-positive and Gram-negative bacteria. This kit combines the advantages of a silica-based resin with magnetic-based separation. The method is based on the lysis of bacterial cells in proximity to magnetic silica particles, and the immobilization of large DNA molecules on the surface of the particles. The resulting DNA:magnetic particle complex is washed to remove impurities and purified genomic DNA is eluted. The procedure for Gram-negative bacteria can be performed in as little as 30 minutes, and genomic DNA from Gram-positive bacteria can be purified in as little as 75 minutes, with much of the procedure being hands-off time. Yields of up to 30 µg of genomic DNA can be achieved with 1 ml of culture. Genomic DNA prepared with MagPrep Bacterial Genomic DNA Kit is suitable for a variety of downstream applications including restriction endonuclease digestion, RFLP analysis, Southern blotting, library construction, and many PCR-based applications.
      Table 1. Strains processed successfully with the
      MagPrep Bacterial Genomic DNA Kit
      Aerococcus viridans
      Agrobacterium radiobacter
      Alcaligenes faecalis
      Bacillus cereus
      Bacillus subtilis
      Citrobacter freundii
      Enterobacter cloacae
      Enterococcus faecalis
      Enterococcus faecium
      Erysipelothrix rhusiopathiae
      Escherichia coli       B
      Escherichia coli K-12
      Klebsiella pneumoniae

      Lactobacillus casei
      Lactococcus lactis
      Leuconostoc mesenteroides
      Listeria innocua       plus 12 subspecies
      Micrococcus luteus
      Proteus vulgaris
      Salmonella enterica
      Serratia marcescens
      Shigella sonnei
      Staphylococcus aureus
      Staphylococcus simulans
      Vibrio parahemolyticus
      Yersinia enterocolitica

      Catalogue Number71256
      Brand Family Novagen®
      References
      Product Information
      •2 × 1 mlMagPrep Silica Particles
      •10 mlBacterial Resuspension Buffer
      •0.1 mlRNAse A Solution
      •20 mgLysozyme, Chicken Egg White
      •0.5 mlProteinase K Solution
      •10 mlMagPrep BG Lysis Buffer
      •2 × 75 mlMagPrep BG Wash Buffer
      •10 mlMagPrep BG Elute Buffer
      DeclarationMagPrep is a registered trademark of EMD Chemicals, Inc.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71256 0