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411466 Mouse Anti-Human IgG₂, Fd Fragment Specific (HP6014)

411466
  
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      Overview

      Replacement Information
      Description
      Catalogue Number411466
      Brand Family Calbiochem®
      References
      ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.4.
      PreservativeNone
      Applications
      Application ReferencesELISA Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
      Application NotesELISA (see comments and application references)
      Application CommentsCan be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: ≤0.06%
      Human IgG2: 100%
      Human IgG3: ≤0.02%
      Human IgG4: ≤0.03%
      Human IgA: ≤0.02%
      Human IgE: ≤0.01%
      Human IgM: ≤0.02%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: <0.01%
      Human IgG2: 100%
      Human IgG3: <0.01%
      Human IgG4: <0.01%
      Human IgA: <0.01%
      Human IgE: <0.001%
      Human IgM: <0.01%
      Biological Information
      CloneHP6014
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Mouse Anti-Human IgG₂, Fd Fragment Specific (HP6014) Certificates of Analysis

      TitleLot Number
      411466

      References

      Reference overview
      Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2007 RFH
      ApplicationELISA (see comments and application references)
      DescriptionPurified mouse monoclonal antibody. Recognizes human IgG2 Fd fragment.
      BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem® technical monograph, The Human IgG Subclasses, is available free of charge.
      HostMouse
      CloneHP6014
      IsotypeIgG₁
      FormLiquid
      FormulationIn PBS, pH 7.4.
      PreservativeNone
      CommentsCan be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.

      Cross-reactivity by ELISA against human myeloma proteins:
      Human IgG1: ≤0.06%
      Human IgG2: 100%
      Human IgG3: ≤0.02%
      Human IgG4: ≤0.03%
      Human IgA: ≤0.02%
      Human IgE: ≤0.01%
      Human IgM: ≤0.02%

      Cross-reactivity by ELISA against chimeric antibodies:
      Human IgG1: <0.01%
      Human IgG2: 100%
      Human IgG3: <0.01%
      Human IgG4: <0.01%
      Human IgA: <0.01%
      Human IgE: <0.001%
      Human IgM: <0.01%
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
      Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
      Jefferis, R., et al. 1986. Immunol. Letters 10, 223.
      Application referencesELISA Hamilton, R.G. 1987. Clin. Chem. 33, 1707.