Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
This item has been added to favorites.
Species
Panel Type
Selected Kit
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
96-Well Plate
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
Qty
Catalogue Number
Ordering Description
Qty/Pack
List Price
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
This item has been added to favorites.
The Product Has Been Added To Your Cart
You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
MultiScreen®HTS-PCF Filter Plates for Solubility Assays
Use of ADME screening to conserve resources in drug discovery and development Weiss, A. and Joseph Machamer American Biotechnology Laboratory, January 2004: 16-17
2004
Screening for pharma gold: Researchers are using membrane technologies for high-throughput ADME analysis Lynch, John,Modern Drug Discovery, January 2004: 27-28 Modern Drug Discovery, January 2004: 27-28
2004
Screening compounds for aqueous solubility: a new automated, high throughput method for solubility determination Weiss, Alan and John Lynch PreClinica, March 2003
2003
FAQ
Question
Answer
What is the well depth and maximum volume capacity of a MultiScreen plate?
The well depth of a 96 well MultiScreen plate is 1.245 cm. The well depth for a 384 well MultiScreen plate is 1.2 cm. The maximum working volume of a 96 well plate is 300 ul. The maximum working volume for a 384 well plate is 100 ul.
Related Products & Applications
Product Families
MultiScreen®HTS Vacuum Manifold
For vacuum filtration of 96 and 384 samples Learn More >>
Titer Plate Shaker
Programmable solid-state design for total convenience Learn More >>
C8875Increased screening throughput and efficiency
Determining a compound's aqueous solubility has become an essential early measurement to make in the drug discovery process. Poor water solubility can cause problems in many different in vitro testing techniques leading to unreliable results and/or reproducibility problems; insoluble precipitates have been shown to cause false positives in bioassays potentially wasting valuable resources. Water solubility also influences absorption and thus can be used to help predict the bioavailability of a molecule.
Protocol
1.
Add compound dissolved in organic solvent to aqueous buffer
2.
Shake for 90 minutes to allow insoluble compound to precipitate
3.
Apply vacuum to filter solution into collection plate. Precipitates remain on membrane. Analyze filtrate in collection plate to quantitate amount of compound still in solution
Performance
Results Correlate to Shake-flask
Fifteen (15) compounds were evaluated by an ASTM shake-flask method and a protocol using the MultiScreenHTS-PCF Filter plate. Shake-flask solubility was determined for solid compounds added to PBS. MultiScreenHTS-PCF Filter plate results were determined for 10 µL of 10 mM DMSO stocks added to 190 µL PBS for a final DMSO concentration of 5%. The maximum concentration on the Y axis is given as 500 µM due to the MultiScreenHTS-PCF Filter plate protocol that has an upper limit for the amount of compound added.
High Drug Recovery
Soluble drugs were dissolved in 5% DMSO/PBS at 1 µM, incubated in the MultiScreenHTS-PCF Filter plate and filtered into a receiver plate. The results are reported as percent drug recovery as determined by radiometric analysis.
Validated for High Drug Recovery
The MultiScreenHTS-PCF Filter plate incorporates low-binding membrane and low-binding plate materials to yield the high drug recovery needed for solubility results. The plate is validated versus a panel of 9 drugs for >80% drug recovery at 10 µM concentration in 5% DMSO/PBS.
Increased Screening Throughput and Efficiency
The MultiScreenHTS-PCF Filter plate is a high throughput, 96-well system to classify or quantify the aqueous solubility of compound libraries stored in solvent as a concentrated solution.
The filtration-based protocol is fast and efficient. The protocol has been validated to screen hundreds of samples per day and saves time and sample over the shake-flask method. Typical MultiScreenHTS-PCF Filter plate analysis time is less than 4 hours and uses <100 µg of sample per analysis. This is an improvement as compared to the shake-flask method that can take several days and require milligrams of sample for each analysis.
MultiScreenHTS-PCF Filter plates are automation compatible. The plates are in compliance with SBS guidelines for easy handling by robotics. They also include a space for barcoding.
)
)
)