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480025 NEMO-Binding Domain Binding Peptide, Cell-Permeable - Calbiochem

NEMO-Binding Domain Binding Peptide, Cell-Permeable, is an Antennapedia-NBD (wild type) fusion peptide that blocks the interaction of NEMO with the IKK (IκB)-kinase complex.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: DRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable

Primary Target: cytokine-induced NF-κB activation and NF-κB-dependent gene expression
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      Overview

      Replacement Information

      Key Spec Table

      Empirical Formula
      C₁₇₀H₂₅₉N₄₉O₄₂S
      Description
      Overview

      This product has been discontinued.



      A cell-permeable Antennapedia-NBD (wild type) fusion peptide that exhibits anti-inflammatory properties. Acts by inhibiting cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Suggested to block the interaction of NEMO with the IKK (IκB)-kinase complex and thereby prevents NF-κB activation. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.
      Catalogue Number480025
      Brand Family Calbiochem®
      SynonymsDRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable
      References
      ReferencesJimi, E., et al. 2004. Nat. Med., 10, 617.
      Siegmund, D., et al. 2001. J. Biol. Chem. 276, 43708.
      May, M.J., et al. 2000. Science 289, 1550.
      Li, Q., et al. 1999. Science 284, 321.
      Product Information
      ATP CompetitiveN
      FormWhite lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₁₇₀H₂₅₉N₄₉O₄₂S
      Chemical formulaC₁₇₀H₂₅₉N₄₉O₄₂S
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      ApplicationNEMO-Binding Domain Binding Peptide, Cell-Permeable, is an Antennapedia-NBD (wild type) fusion peptide that blocks the interaction of NEMO with the IKK (IκB)-kinase complex.
      Biological Information
      Primary Targetcytokine-induced NF-κB activation and NF-κB-dependent gene expression
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Peptide SequenceH-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu-OH
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      480025 0

      Documentation

      NEMO-Binding Domain Binding Peptide, Cell-Permeable - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      NEMO-Binding Domain Binding Peptide, Cell-Permeable - Calbiochem Certificates of Analysis

      TitleLot Number
      480025

      References

      Reference overview
      Jimi, E., et al. 2004. Nat. Med., 10, 617.
      Siegmund, D., et al. 2001. J. Biol. Chem. 276, 43708.
      May, M.J., et al. 2000. Science 289, 1550.
      Li, Q., et al. 1999. Science 284, 321.

      Brochure

      Title
      Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

      Citations

      Title
    • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-May-2011 JSW
      SynonymsDRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable
      DescriptionA cell-permeable Antennapedia-NBD (NEMO binding domain) (wild type) fusion peptide that exhibits anti-inflammatory activity in mouse model of acute inflammation. NBD is an amino terminal α-helical region of the NEMO (NF-κB essential modifier; IKKγ) associated with a carboxyl-terminal segment of IKKα and IKKβ. This peptide blocks the association of NEMO with the IKK complex and prevents NF-κB activation. NEMO is also reported to suppress the intrinsic basal activity of the IKK complex. Its anti-inflammatory properties are attributed to the inhibition of cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Does neither affect the TNF-α-induced phosphorylation of c-Jun nor the DNA binding of Oct-1 transcription factor.
      FormWhite lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₇₀H₂₅₉N₄₉O₄₂S
      Peptide SequenceH-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu-OH
      Purity≥95% by HPLC
      SolubilityDMSO (50 mg/ml). Note: This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations. However, sufficient material will stay in the medium to permeate cells.
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesJimi, E., et al. 2004. Nat. Med., 10, 617.
      Siegmund, D., et al. 2001. J. Biol. Chem. 276, 43708.
      May, M.J., et al. 2000. Science 289, 1550.
      Li, Q., et al. 1999. Science 284, 321.
      Citation
    • Soonkyu Chung, et al. (2005) Conjugated linoleic acid promotes human adipocyte insulin resistance through NF-κB-dependent cytokine production. Journal of Biological Chemistry 280, 38445-38456.
      		•