481418 Sigma-AldrichNEMO NOA Peptide, Cell-Permeable, A-UBI - Calbiochem

This product has been discontinued.

A cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (K_d = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC₅₀ = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC₅₀ = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.