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105226 New fuchsin (C.I. 42520)

for microscopy Certistain®

New fuchsin (C.I. 42520) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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105226
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Overview

Replacement Information
Description
Catalogue Number105226
DescriptionNew fuchsin (C.I. 42520)
OverviewThe dry dye New fuchsin (C.I. 42520) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for staining of histological tissue sections or other samples of human origin according to Ziehl-Neelsen, Gram staining as well as in combination with Schiff’s reagent. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. New fuchsin belongs to the group of triaminotriphenylmethane dyes.
The dye is used in Ziehl-Neelsen and Gram staining as well as for methods with Schiff’s reagent.
The cell wall of acid-fast bacteria (AFB) has a high proportion of wax and lipids and hence absorbs dyes only very slowly. As a measure to enhance the absorption of the fuchsin dye and thus the formation of the mycolate-fuchsin complex in the cell wall, the carbolfuchsin solution applied to the specimen is normally heated to evaporation point. If the Kinyoun carbolfuchsin solution is used, the heating of the solution is dispensable. Consequently, the release of hazardous phenolic vapors will be avoided.
Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol® also has a disinfectant effect, with the result that any microorganisms that are present are killed off. It is registered as IVD and certified as CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
CAS number3248-91-7
EC number221-831-7
Hill FormulaC₂₂H₂₄ClN₃
Molar Mass365.91 g/mol
HS Code3212 90 00
Structure formula ImageStructure formula Image
Quality LevelMQ400
Applications
Applicationfor microscopy Certistain®
Biological Information
Physicochemical Information
pH value3 - 4 (10 g/l, H₂O, 20 °C)
Bulk density450 kg/m3
Solubility10 g/l
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H315: Causes skin irritation.
H319: Causes serious eye irritation.
H335: May cause respiratory irritation.
H350: May cause cancer.
H410: Very toxic to aquatic life with long lasting effects.
Precautionary Statement(s)P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P273: Avoid release to the environment.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308 + P313: IF exposed or concerned: Get medical advice/ attention.
Signal WordDanger
RTECSCX9825000
Storage class6.1C Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGKWGK 3 highly hazardous to water
Disposal9
Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
Safety Information
Categories of dangercarcinogenic
Product Usage Statements
Storage and Shipping Information
StorageStore at +2°C to +30°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 3077 , 9, III
Declaration (transport by air) IATA-DGRUN 3077 , 9, III
Declaration (transport by sea) IMDG-CodeUN 3077 , 9, III, Marine Pollutant: P
Supplemental Information
Specifications
Dye content (spectrophotometrically)≥ 75 %
Identity (UV/VIS-Spectrum)passes test
Absorption maximum λ max (ethanol 50 %)553 - 556 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)1810 - 2430
TLC-Testpasses test
Loss on drying (110 °C)≤ 10 %
Suitability for microscopypasses test