72633 Sigma-AldrichNovaQUANT™ Coli qPCR Kit

Product Description

The NovaQUANT™ Coli qPCR kit is designed to quantify the absolute amount of E. coli DNA present in a sample. The assay has been shown to accurately detect DNA from several E. coli strains present in many sample matrices including protein, nucleic acid and polysaccharide preparations.

The kit contains a set of oligonucleotide primers developed for SYBR^® Green-based qPCR detection. The NovaQUANT Coli qPCR primers were designed to detect a high-copy number of a highly-conserved, E. coli ribosomal RNA gene; this feature allows for the accurate detection of trace amounts of bacterial DNA, typically in the low femtogram range.

Panel Components

• 200 µl         NovaQUANT Coli qPCR Primer Mix (2 µM)

• 100 ng         NovaQUANT Coli Control DNA (10 ng/µl)

Storage: Store all components at -20 °C. This kit is stable for at least one year at the recommended storage temperature.