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492899 Nuclease, Staphylococcus aureus

Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3'-phosphate groups.

Nuclease, Staphylococcus aureus MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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492899
  
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      Overview

      Replacement Information
      Description
      OverviewNative Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.
      Catalogue Number492899
      Brand Family Calbiochem®
      References
      ReferencesKrupp, G., and Gross, H.J. 1979. Nucleic Acids Res. 6, 3481.
      Product Information
      CAS number9013-53-0
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.
      EC number3.1.31.1
      FormLyophilized white to off-white solid
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥15,000 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      492899 0

      Documentation

      Nuclease, Staphylococcus aureus SDS

      Title

      Safety Data Sheet (SDS) 

      Nuclease, Staphylococcus aureus Certificates of Analysis

      TitleLot Number
      492899

      References

      Reference overview
      Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res. 6, 3481.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2019 JSW
      DescriptionNative Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.
      FormLyophilized white to off-white solid
      CAS number9013-53-0
      EC number3.1.31.1
      Specific activity≥15,000 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.
      SolubilityAqueous buffers (1 mg/ml)
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesKrupp, G., and Gross, H.J. 1979. Nucleic Acids Res. 6, 3481.