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526552 PI 3-Kα, p110α/p85α, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
526552
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Recombinant, human phosphoinositide 3-kinase p110α fused at the N-terminus to a His•Tag®sequence and human p85α co-expressed in S. frugiperda insect cells. The active complex composed of the catalytic p110α subunit and the regulatory p85α subunit is useful for the study of activity regulation and for inhibitor screening.
      Catalogue Number526552
      Brand Family Calbiochem®
      Application Data
      The activity was determined using phosphatidylinositol as the substrate and 1 µM ATP.
      References
      ReferencesZhao, J. J., et.al. 2006. Science 365, 52.
      Meier, T.I., et.al. 2004. Protein Expr Purif. 35, 21.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to phosphatidylinositol per min at room temperature.
      FormLiquid
      FormulationIn 300 mM NaCl, 50 mM Tris-HCl, pH 7.9.
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥500 units/mg
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      526552 0

      Documentation

      References

      Reference overview
      Zhao, J. J., et.al. 2006. Science 365, 52.
      Meier, T.I., et.al. 2004. Protein Expr Purif. 35, 21.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-March-2009 JSW
      Application Data
      The activity was determined using phosphatidylinositol as the substrate and 1 µM ATP.
      DescriptionRecombinant, human phosphoinositide 3-kinase p110α fused at the N-terminus to a His•Tag®sequence and human p85α co-expressed in S. frugiperda insect cells. The active complex composed of the catalytic p110α subunit and the regulatory p85α subunit is useful for the study of activity regulation and for inhibitor screening.
      FormLiquid
      FormulationIn 300 mM NaCl, 50 mM Tris-HCl, pH 7.9.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Kinase Activity Assay
      Required Materials • Assay Buffer: 50 mM Tris-HCl, 10 mM NaCl, 10 mM DTT, 0.05% Brij®-35 detergent, pH 7.5 • Phosphatidylserine (PS): 10 mg/ml PS in chloroform • Phosphatidylcholine (PC): 10 mg/ml PC in chloroform • Phosphatidylethanolamine (PE): 10 mg/ml PE in chloroform • Phosphatidylinositol (PI): 1 mg/ml PI in chloroform • PI 3-Kα, p110α/p85α: 0.33 mg/ml, diluted to 0.01-0.05 mg/ml in Assay Buffer • ATP/MgCl2 Mix: 5 µM ATP, 100 mM MgCl2 • EasyLite™ Kinase Reagent: available from Perkin Elmer • 96-well plate Preparation of Reagents • Lipid Mix: for each assay, in a glass vial, mix 0.5 µl PC, 1.2 µl PS, 1.1 µl PE, 1.5 µl PI. Let it stand for ~30 min until the chloroform is evaporated. Reconstitute with 30 &µl Assay Buffer without Brij®-35 detergent. Activity Assay 1. To designated wells, add 30 µl Lipid Mix and 10 µl PI 3-Kα (100-500 ng/assay). Incubate for 5 min at 22°C. 2. Add 10 µl ATP/MgCl2 mix and incubate for 30 min at 22°C. 3. Add 50 µl pre-warmed EasyLite™ Kinase Reagent. 4. Measure activity using a luminometer.
      Purity≥90% by SDS-PAGE
      Specific activity≥500 units/mg
      Unit definitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to phosphatidylinositol per min at room temperature.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesZhao, J. J., et.al. 2006. Science 365, 52.
      Meier, T.I., et.al. 2004. Protein Expr Purif. 35, 21.