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539677 PKCθ, His•Tag®, Human, Recombinant, S. frugiperda

539677
  
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      Overview

      Replacement Information
      Description
      OverviewFull-length, recombinant, human PKC fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PKC is a calcium-independent PKC, which is expressed in T lymphocytes and muscle. PKC is reported to be required for T cell activation, proliferation, and cytokine production.
      Catalogue Number539677
      Brand Family Calbiochem®
      SynonymsProtein Kinase Cθ
      References
      ReferencesAltman, A. and Villalba, M. 2002. J. Biochem. 132, 841.
      Villalba, M., et al. 2001. J. Immunol. 166, 5955.
      Baier, G., et al. 1994. Eur. J. Biochem. 225, 195.
      Baier, G., et al. 1993. J. Biol. Chem. 268, 4997.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKC<sub>ε</sub> Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5
      EC number2.7.1
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM HEPES, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% NP-40, pH 7.5.
      Applications
      Biological Information
      Primary TargetPKCθ
      Purity≥90% by SDS-PAGE
      Specific Activity≥2500 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PKCθ, His•Tag®, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      PKCθ, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      539677

      References

      Reference overview
      Altman, A. and Villalba, M. 2002. J. Biochem. 132, 841.
      Villalba, M., et al. 2001. J. Immunol. 166, 5955.
      Baier, G., et al. 1994. Eur. J. Biochem. 225, 195.
      Baier, G., et al. 1993. J. Biol. Chem. 268, 4997.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-November-2008 JSW
      SynonymsProtein Kinase Cθ
      DescriptionFull-length, recombinant, human PKC fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PKC is a calcium-independent PKC, which is expressed in T lymphocytes and muscle. PKC is reported to be required for T cell activation, proliferation, and cytokine production.
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM HEPES, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% NP-40, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      20 mM HEPES, pH 7.5, 10 mM MgCl2, 100 µM EGTA, 100 µg/ml PKCε substrate (Cat. No. 539562), 100 µM ATP, 200 µg/ml phosphatidylserine, 20 µg/ml diacylglycerol, 0.03% Triton® X-100, trace [32P]-γ-ATP, 5 µl enzyme in a total reaction volume of 50 µl; incubate at 30°C for the desired time.
      EC number2.7.1
      Purity≥90% by SDS-PAGE
      Specific activity≥2500 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKCε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesAltman, A. and Villalba, M. 2002. J. Biochem. 132, 841.
      Villalba, M., et al. 2001. J. Immunol. 166, 5955.
      Baier, G., et al. 1994. Eur. J. Biochem. 225, 195.
      Baier, G., et al. 1993. J. Biol. Chem. 268, 4997.