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524576 Phosphatase, Alkaline, Calf Intestine, Molecular Biology Grade

Dephosphorylation of nucleic acids

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CIAP

524576
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.

      Please refer to our complete listing of alkaline phosphatases for possible alternatives. Please read the alternative product documentation carefully and contact technical service if you need additional information.





      Native alkaline phosphatase from calf intestine. Calf Intestine Alkaline Phosphatase catalyzes the hydrolysis of 5'-terminal phosphates of DNA, RNA, and deoxy- and ribonucleoside triphosphates. This preparation is suitable for dephosphorylation of vector DNA to prevent “empty vector” religation in cloning experiments, and for removal of 5'-phosphate groups in preparation for labeling with polynucleotide kinase.
      Catalogue Number524576
      Brand Family Calbiochem®
      SynonymsCIAP
      References
      ReferencesMoessner, E., et al. 1980. J. Physiol. Chem. 361, 543.
      Product Information
      CAS number9001-78-9
      Activity≥1000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of <i>p</i>NPP per min at 37°C, pH 9.8.
      EC number3.1.3.1
      FormLiquid
      FormulationIn 25 mM Tris-HCl, 1 mM MgCl₂, 100 µM ZnCl₂, 50% glycerol, pH 7.6.
      Applications
      Biological Information
      Specific Activity≥2000 units/mg
      Physicochemical Information
      ContaminantsDNases, RNases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      524576 0

      Documentation

      Phosphatase, Alkaline, Calf Intestine, Molecular Biology Grade Certificates of Analysis

      TitleLot Number
      524576

      References

      Reference overview
      Moessner, E., et al. 1980. J. Physiol. Chem. 361, 543.

      Brochure

      Title
      Protein Phosphatases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2008 RFH
      SynonymsCIAP
      DescriptionNative alkaline phosphatase from calf intestine. Calf Intestine Alkaline Phosphatase catalyzes the hydrolysis of 5'-terminal phosphates of DNA, RNA, and deoxy- and ribonucleoside triphosphates. This preparation is suitable for dephosphorylation of vector DNA to prevent “empty vector” religation in cloning experiments, and for removal of 5'-phosphate groups in preparation for labeling with polynucleotide kinase. Suitable for use in reactions containing nucleotide sugars. Has an optimal pH of 8.0-10.5.
      FormLiquid
      FormulationIn 25 mM Tris-HCl, 1 mM MgCl₂, 100 µM ZnCl₂, 50% glycerol, pH 7.6.
      CAS number9001-78-9
      EC number3.1.3.1
      ContaminantsDNases, RNases: none detected
      Specific activity≥2000 units/mg
      Activity≥1000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of pNPP per min at 37°C, pH 9.8.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesMoessner, E., et al. 1980. J. Physiol. Chem. 361, 543.