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PC449 PhosphoDetect™ Anti-Tie2 (Ab-1) (pTyr1102/1108) Rabbit pAb

This PhosphoDetect™ Anti-Tie2 (Ab-1) (pTyr1102/1108) Rabbit pAb is validated for use in ELISA, Paraffin Sections for the detection of Tie2 (Ab-1) (pTyr1102/1108).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Angiopoietin Receptor, Anti-Tek

PC449
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~140-145 Tie2 protein phosphorylated at Tyr1102 and Tyr1108 in colon carcinoma tissue.
      Catalogue NumberPC449
      Brand Family Calbiochem®
      SynonymsAnti-Angiopoietin Receptor, Anti-Tek
      Application Data
      Detection of Tie-2, phosphorylated at Tyr1102/1108, by staining paraffin sections. Sample: Human colon carcinoma. Primary antibody: PhosphoDetect™ Anti-Tie2 (Ab-1) Rabbit pAb (Cat. No. PC449) (1:5000). Detection: DAB with methyl green counterstain.
      References
      ReferencesHayes, A. J., et al. 1999. Microvasc. Res. 58, 2224.
      Jones, N., et al. 1999. J. Biol. Chem. 274, 30896.
      Valenzuela, D. M., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1904.
      Jones, N., and Dumont, D. J. 1998. Oncogene 17, 1097.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlColon carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Paraffin Sections
      Application NotesELISA (see comments)
      Paraffin Sections (1:2000-1:5000, heat pre-treatment required)
      Application CommentsCan be used in conjunction with a biotinylated phosphopeptide to measure the level of Tie2 activity by competition ELISA. Antibody should be titrated for optimal results in individual system.
      Biological Information
      Immunogena synthetic phosphopeptide [EERRTpYVNTTLpYEK(C)] corresponding to amino acids 1097-1110 of human Tie2, where the K in position 1100 of the native sequence has been substituted with R
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC449 0

      Documentation

      PhosphoDetect™ Anti-Tie2 (Ab-1) (pTyr1102/1108) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Tie2 (Ab-1) (pTyr1102/1108) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC449

      References

      Reference overview
      Hayes, A. J., et al. 1999. Microvasc. Res. 58, 2224.
      Jones, N., et al. 1999. J. Biol. Chem. 274, 30896.
      Valenzuela, D. M., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1904.
      Jones, N., and Dumont, D. J. 1998. Oncogene 17, 1097.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-October-2007 RFH
      SynonymsAnti-Angiopoietin Receptor, Anti-Tek
      ApplicationELISA (see comments)
      Paraffin Sections (1:2000-1:5000, heat pre-treatment required)
      Application Data
      Detection of Tie-2, phosphorylated at Tyr1102/1108, by staining paraffin sections. Sample: Human colon carcinoma. Primary antibody: PhosphoDetect™ Anti-Tie2 (Ab-1) Rabbit pAb (Cat. No. PC449) (1:5000). Detection: DAB with methyl green counterstain.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1102 and Tyr1108.
      BackgroundAngiopoietin-1 (Ang1) and its naturally occurring inhibitor, angiopoietin-2 (Ang2) exert their effects by inducing autophosphorylation of their signaling receptor Tie2 (also known as Tek). Phosphorylation of the receptor occurs during vasculogenesis and is required for its activity in regulating endothelial cell proliferation, differentiation and guiding the proper patterning of endothelial cells during blood vessel formation. Tie-2 is a 140 kDa transmembrane receptor-tyrosine kinase that is expressed by endothelial cells and primitive hematopoietic tissues. Tie2/Tek has been shown to be critical for endothelial cell survival, which is evidenced in part by the constitutive phosphorylation that is observed in quiescent endothelium. More recently a multifunction docking site has been identified on the receptor (Tyr1100) that serves as a binding site for other partners including Grb2, Grb7, and p85 and acting via an SH2 domain interaction.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide [EERRTpYVNTTLpYEK(C)] corresponding to amino acids 1097-1110 of human Tie2, where the K in position 1100 of the native sequence has been substituted with R
      IsotypeIgG
      Specieshuman
      Positive controlColon carcinoma tissue
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsCan be used in conjunction with a biotinylated phosphopeptide to measure the level of Tie2 activity by competition ELISA. Antibody should be titrated for optimal results in individual system.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHayes, A. J., et al. 1999. Microvasc. Res. 58, 2224.
      Jones, N., et al. 1999. J. Biol. Chem. 274, 30896.
      Valenzuela, D. M., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1904.
      Jones, N., and Dumont, D. J. 1998. Oncogene 17, 1097.

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