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PC450 PhosphoDetect™ Anti-Tie2 (pTyr 1108/1117) (Ab-2) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Angiopoietin Receptor, Anti-Tek

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1108 and Tyr1117 in colon carcinoma tissue.
      Catalogue NumberPC450
      Brand Family Calbiochem®
      SynonymsAnti-Angiopoietin Receptor, Anti-Tek
      Application Data
      Detection of human Tie2 phosphorylated on Tyr1108/1117, by staining paraffin sections. Sample: Colon carcinoma. Primary antibody: PhosphoDetect™ Anti-Tie2 (pTyr1108/1117) (Ab-2) Rabbit pAb (Cat. No. PC450) (1:5000). Detection: DAB with methyl green counterstain.
      References
      ReferencesHayes, A.J., et al. 1999. Microvasc. Res. 58, 2224.
      Jones, N., et al. 1999. J. Biol. Chem. 274, 30896.
      Valenzuela, D.M., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1904.
      Jones, N. and Dumont, D.J. 1998. Oncogene 17, 1097.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlColon carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Paraffin Sections
      Application NotesELISA (see comments)
      Paraffin Sections (1:2000-1:5000, no pre-treatment required)
      Application CommentsThis antibody can be used in conjunction with a biotinylated phosphopeptide to measure the level of Tie2 activity by competition ELISA. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide [pYEKFTpYAGID(C)] corresponding to amino acids surrounding the Tyr1108/1117 phosphorylation sites 1108-1117 of human Tie2
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC450 0

      Documentation

      PhosphoDetect™ Anti-Tie2 (pTyr 1108/1117) (Ab-2) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Hayes, A.J., et al. 1999. Microvasc. Res. 58, 2224.
      Jones, N., et al. 1999. J. Biol. Chem. 274, 30896.
      Valenzuela, D.M., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1904.
      Jones, N. and Dumont, D.J. 1998. Oncogene 17, 1097.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-October-2007 RFH
      SynonymsAnti-Angiopoietin Receptor, Anti-Tek
      ApplicationELISA (see comments)
      Paraffin Sections (1:2000-1:5000, no pre-treatment required)
      Application Data
      Detection of human Tie2 phosphorylated on Tyr1108/1117, by staining paraffin sections. Sample: Colon carcinoma. Primary antibody: PhosphoDetect™ Anti-Tie2 (pTyr1108/1117) (Ab-2) Rabbit pAb (Cat. No. PC450) (1:5000). Detection: DAB with methyl green counterstain.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~140-145 Tie2 protein phosphorylated at Tyr1108 and Tyr1117.
      BackgroundAngiopoietin-1 (Ang1) and its naturally occurring inhibitor, angiopoietin-2 (Ang2) exert their effects by inducing autophosphorylation of their signaling receptor Tie2 (also known as Tek). Phosphorylation of the receptor occurs during vasculogenesis and is required for its activity in regulating endothelial cell proliferation, differentiation and guiding the proper patterning of endothelial cells during blood vessel formation. Tie-2 is a ~140 kDa transmembrane receptor-tyrosine kinase that is expressed by endothelial cells and primitive hematopoietic tissues. Tie2/Tek has been shown to be critical for endothelial cell survival, which is evidenced in part by the constitutive phosphorylation that is observed in quiescent endothelium. More recently a multifunction docking site has been identified on the receptor (Tyr1100in mouse or Tyr1102in human) that serves as a binding site for other partners including Grb2, Grb7, and p85 and acting via an SH2 domain interaction.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide [pYEKFTpYAGID(C)] corresponding to amino acids surrounding the Tyr1108/1117 phosphorylation sites 1108-1117 of human Tie2
      IsotypeIgG
      Specieshuman
      Positive controlColon carcinoma tissue
      FormLiquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsThis antibody can be used in conjunction with a biotinylated phosphopeptide to measure the level of Tie2 activity by competition ELISA. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesHayes, A.J., et al. 1999. Microvasc. Res. 58, 2224.
      Jones, N., et al. 1999. J. Biol. Chem. 274, 30896.
      Valenzuela, D.M., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 1904.
      Jones, N. and Dumont, D.J. 1998. Oncogene 17, 1097.

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