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662036 Pro-SUMO-1, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sentrin, Ubiquitin-like protein SMT3C

662036
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human pro-SUMO-1 expressed in E. coli. SUMOylation results in altered subcellular localization of target proteins involved in cell cycle, transcription, cell death, and stress response, as well as membrane-associated receptors, and viral proteins. Pro-SUMOs are processed by SENPs (endopeptidase cleavage) to remove short C-terminal extensions revealing the Gly-Gly motif that is essential for substrate conjugation to target proteins. Pro-SUMO-1 (101 amino acids) is the inactive precursor of SUMO-1 (97 amino acids) and is processed at the C-terminus by SENPs. The protein can be used as a substrate for SENPs or as a negative control in sumoylation reactions.
      Catalogue Number662036
      Brand Family Calbiochem®
      SynonymsSentrin, Ubiquitin-like protein SMT3C
      References
      ReferencesDrag, M., et al. 2008. Biochem J. 409, 461.
      Mikolajczyk, J., et al. 2007. J. Biol. Chem. 282, 26217.
      Hochstrasser, M., 2001. Cell 107, 5.
      Wilson, V.G., and Rangasam, D., 2001. Exp. Cell Res. 271, 57.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM HEPES, 1 mM DTT, 10% glycerol, pH 8.0.
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      662036 0

      Documentation

      References

      Reference overview
      Drag, M., et al. 2008. Biochem J. 409, 461.
      Mikolajczyk, J., et al. 2007. J. Biol. Chem. 282, 26217.
      Hochstrasser, M., 2001. Cell 107, 5.
      Wilson, V.G., and Rangasam, D., 2001. Exp. Cell Res. 271, 57.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-April-2009 JSW
      SynonymsSentrin, Ubiquitin-like protein SMT3C
      DescriptionFull-length, recombinant, human pro-SUMO-1 expressed in E. coli. SUMOylation results in altered subcellular localization of target proteins involved in cell cycle, transcription, cell death, and stress response, as well as membrane-associated receptors, and viral proteins. Pro-SUMOs are processed by SENPs (endopeptidase cleavage) to remove short C-terminal extensions revealing the Gly-Gly motif that is essential for substrate conjugation to target proteins. Pro-SUMO-1 (101 amino acids) is the inactive precursor of SUMO-1 (97 amino acids) and is processed at the C-terminus by SENPs. The protein can be used as a substrate for SENPs or as a negative control in sumoylation reactions.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM HEPES, 1 mM DTT, 10% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Typical concentration for in vitro assays: 10-50 µM
      Purity≥95% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesDrag, M., et al. 2008. Biochem J. 409, 461.
      Mikolajczyk, J., et al. 2007. J. Biol. Chem. 282, 26217.
      Hochstrasser, M., 2001. Cell 107, 5.
      Wilson, V.G., and Rangasam, D., 2001. Exp. Cell Res. 271, 57.