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PAT001-100UG Protein A-Turbo - Proximity Biotinylation Enzyme

Proximity Biotinylation - Protein A Fusion Enzyme, Cat. No. PAT001. Proximity biotinylation is a tool for probing protein-protein interactions. It is used in Affinity Binding Assay, Immunocytochemistry, Proximity Ligation Assay, IP, and Mass Spec.

PAT001-100UG
100 μg  Liquid
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      Overview

      Replacement Information
      Description
      Catalogue NumberPAT001-100UG
      DescriptionProtein A-Turbo - Proximity Biotinylation Enzyme
      Alternate Names
      • ProteinA-Turbo ID
      Background InformationProtein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.
      References
      Product Information
      FormatPurified
      PresentationPurified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.
      Quality LevelMQ200
      Applications
      ApplicationProximity Biotinylation - Protein A Fusion Enzyme, Cat. No. PAT001. Proximity biotinylation is a tool for probing protein-protein interactions. It is used in Affinity Binding Assay, Immunocytochemistry, Proximity Ligation Assay, IP, and Mass Spec.
      Key Applications
      • Immunofluorescence
      • Chromatography
      • Mass Spectrometry
      • Immunoprecipitation
      Application Notes- Proximity protein labeling
      - Cell Biology
      - Histology
      - Fixed cell microscopy
      - Tissue staining
      - Immunofluorescence

      -Liquid Chromatography

      - Mass Spectrometry

      -Immunoprecipitation
      Biological Information
      Concentration0.7 mg/mL
      SpecificityProtein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEnzyme was tested for proximity biotinylation in cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -65°C to -96°C.
      Packaging Information
      Material Size100 μg
      Material PackageLiquid
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PAT001-100UG 04065270500014

      Documentation

      Protein A-Turbo - Proximity Biotinylation Enzyme Certificates of Analysis

      TitleLot Number
      Protein A-Turbo - Proximity Biotinylation Enzyme - 4258338 4258338
      Protein A-Turbo - Proximity Biotinylation Enzyme - 4331560 4331560
      Protein A-Turbo - Proximity Biotinylation Enzyme - 4334381 4334381
      Protein A-Turbo - Proximity Biotinylation Enzyme - 4335317 4335317
      Protein A-Turbo - Proximity Biotinylation Enzyme - Q4083389 Q4083389
      Protein A-Turbo - Proximity Biotinylation Enzyme - Q4083395 Q4083395

      References

      Reference overviewPub Med ID
      Off-the-shelf proximity biotinylation for interaction proteomics
      Santos-Barriopedro I, et al.
      Nature Communications  12  1270-1281  2021

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