Millipore Sigma Vibrant Logo

527151 Protein Kinase C Selectide™ Substrate

Protein Kinase C Selectide™ Substrate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Kinase C Selectide™ Substrate, Neurogranin 28-43, AAKIQASFRGHMARKK

527151
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      OverviewProtein Kinase C Selectide™ Substrate has been developed to overcome difficulties that can arise from using non-specific substrates when assaying PKC activity in crude cell extracts. Structurally, Protein Kinase C Selectide™ Substrate is based on the sequence of neurogranin, a naturally-occurring PKC substrate. Protein Kinase C Selectide™ Substrate (Km = 100 - 150 nM) allows the measurement of PKC activity, even in crude cell extracts.
      Catalogue Number527151
      Brand Family Calbiochem®
      SynonymsProtein Kinase C Selectide™ Substrate, Neurogranin 28-43, AAKIQASFRGHMARKK
      References
      ReferencesChang, D.K., et al. 1997. Biophys. J. 72, 554. Chen, S.J., et al. 1993. Biochemistry 32, 1032. Baudier, J., et al. 1989. J. Biol. Chem. 264, 1824.
      Product Information
      FormWhite solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₇₈H₁₃₄N₂₈O₁₉S
      Chemical formulaC₇₈H₁₃₄N₂₈O₁₉S
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Peptide SequenceH-Ala-Ala-Lys-Ile-Gln-Ala-Ser-Phe-Arg-Gly-His-Met-Ala-Arg-Lys-Lys-OH
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      527151 0

      Documentation

      Protein Kinase C Selectide™ Substrate Certificates of Analysis

      TitleLot Number
      527151

      References

      Reference overview
      Chang, D.K., et al. 1997. Biophys. J. 72, 554. Chen, S.J., et al. 1993. Biochemistry 32, 1032. Baudier, J., et al. 1989. J. Biol. Chem. 264, 1824.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-August-2008 RFH
      SynonymsProtein Kinase C Selectide™ Substrate, Neurogranin 28-43, AAKIQASFRGHMARKK
      DescriptionMeasurement of PKC activity is typically assayed in vitro using substrates, such as purified enzymes, naturally occurring proteins (i.e. histone H1), cell extracts or synthetic peptides. PKC Selectide™ Substrate has been developed to overcome difficulties that can arise from using non-specific substrates when assaying PKC activity in crude cell extracts. Structurally, PKC Selectide™ Substrate corresponds to residues 28-43 of neurogranin, a naturally occurring PKC substrate. PKC Selectide™ Substrate allows selective measurement of PKC versus other kinases, even in crude cell extracts (Km = 100-150 nM).
      FormWhite solid
      FormulationSupplied as a trifluoroacetate salt.
      Chemical formulaC₇₈H₁₃₄N₂₈O₁₉S
      Peptide SequenceH-Ala-Ala-Lys-Ile-Gln-Ala-Ser-Phe-Arg-Gly-His-Met-Ala-Arg-Lys-Lys-OH
      Purity≥95% by HPLC
      SolubilityH₂O (25 mg/ml)
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChang, D.K., et al. 1997. Biophys. J. 72, 554. Chen, S.J., et al. 1993. Biochemistry 32, 1032. Baudier, J., et al. 1989. J. Biol. Chem. 264, 1824.