ECM510 Sigma-AldrichQCM Chemotaxis Cell Migration Assay, 96-well (8 µm), fluorimetric
The QCM 8 uM 96-well Migration Assay utilizes a 8 um pore size, which is appropriate for leukocyte migration.
More>> The QCM 8 uM 96-well Migration Assay utilizes a 8 um pore size, which is appropriate for leukocyte migration. Less<<Recommended Products
Overview
Replacement Information |
---|
Key Spec Table
Key Applications | Detection Methods |
---|---|
ACT | Fluorescent |
Description | |
---|---|
Catalogue Number | ECM510 |
Brand Family | Chemicon® |
Trade Name |
|
Description | QCM Chemotaxis Cell Migration Assay, 96-well (8 µm), fluorimetric |
Overview | Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here Introduction Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-migrated cells on the top side of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable. The Chemicon QCM™ 96-well Migration Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. In the Chemicon QCM™ 96-well Migration Assay, migratory cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQuant GR dye (Molecular Probes). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids. The Chemicon QCM™ 96-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells. The Chemicon QCM™ 96-well Migration Assay utilizes an 8 μm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis. In addition, Chemicon also provides QCM™ 24-well insert cell migration assay systems, CytoMatrix™ Cell Adhesion strips coated with ECM proteins or anti integrin antibodies, and QuantiMatrix™ ECM protein ELISA kits. Application: The Chemicon QCM™ 96-well Migration Assay is ideal for the study of chemotaxis cell migration. The quantitative nature of this assay is especially useful for large scale screening of pharmacological agents. The 8 μm pore size of this assay's Boyden chambers is appropriate for migration studies of most cell types. Each kit provides sufficient materials for the evaluation of 96 samples. The Chemicon QCM™ 96-well Migration Assay is intended for research use only; not for diagnostic applications. |
Materials Required but Not Delivered | 1. Precision pipettes: sufficient for aliquoting cells. 2. Harvesting buffer: EDTA or trypsin cell detachment buffer. Suggested formulations include a) 2 mM EDTA/PBS, b) 0.05% trypsin in Hanks Balanced Salt Solution (HBSS) containing 25 mM HEPES, or other cell detachment formulations as optimized by individual investigators. Note: Trypsin cell detachment buffer maybe required for difficult cell lines. Allow sufficient time for cell receptor recovery. 3. Tissue culture growth medium appropriate for subject cells, such as DMEM containing 10% FBS. 4. Chemoattractants (eg. 10% FBS) or pharmacological agents for addition to culture medium, if screening is desired. 5. Quenching Medium: serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media), containing 5% BSA. Note: Quenching Medium must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in the harvesting buffer. 6. Sterile PBS or HBSS to wash cells. 7. Distilled water. 8. Low speed centrifuge and tubes for cell harvesting. 9. CO2 incubator appropriate for subject cells. 10. Hemocytometer or other means of counting cells. 11. Trypan blue or equivalent viability stain. 12. Fluorescence plate reader. 13. Sterile cell culture hood. |
References |
---|
Product Information | |
---|---|
Components |
|
Detection method | Fluorescent |
HS Code | 3822 19 90 |
Quality Level | MQ100 |
Applications | |
---|---|
Application | The QCM 8 uM 96-well Migration Assay utilizes a 8 um pore size, which is appropriate for leukocyte migration. |
Key Applications |
|
Biological Information | |
---|---|
Species Reactivity |
|
Physicochemical Information |
---|
Dimensions |
---|
Materials Information |
---|
Toxicological Information |
---|
Safety Information according to GHS |
---|
Safety Information |
---|
Storage and Shipping Information |
---|
Packaging Information | |
---|---|
Material Size | 1 plate |
Material Package | 96 wells |
Transport Information |
---|
Supplemental Information |
---|
Specifications |
---|
Global Trade Item Number | |
---|---|
Catalogue Number | GTIN |
ECM510 | 04053252506604 |
Documentation
QCM Chemotaxis Cell Migration Assay, 96-well (8 µm), fluorimetric SDS
Title |
---|
References
Reference overview | Application | Pub Med ID |
---|---|---|
Meteorin is a chemokinetic factor in neuroblast migration and promotes stroke-induced striatal neurogenesis. Zhaolu Wang,Nuno Andrade,Malene Torp,Somsak Wattananit,Andreas Arvidsson,Zaal Kokaia,Jesper Roland Jørgensen,Olle Lindvall Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 32 2012 Show Abstract | 22044868 | |
In vitro reconstitution of human kidney structures for renal cell therapy. Nadia K Guimaraes-Souza,Liliya M Yamaleyeva,Tamer Aboushwareb,Anthony Atala,James J Yoo Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 27 2012 Show Abstract | 22287659 | |
Coupling in vitro and in vivo paradigm reveals a dose dependent inhibition of angiogenesis followed by initiation of autophagy by C6-ceramide. Rishipal R Bansode,Mohamed Ahmedna,Kurt R Svoboda,Jack N Losso International journal of biological sciences 7 2011 Show Abstract Full Text Article | 21647331 | |
Breast cancer cell surface annexin II induces cell migration and neoangiogenesis via tPA dependent plasmin generation. Meena Sharma,Robert T Ownbey,Mahesh C Sharma Experimental and molecular pathology 88 2010 Show Abstract | 20079732 | |
Novel mechanism for obesity-induced colon cancer progression. Janette M Birmingham,Julia V Busik,Fay M Hansen-Smith,Jenifer I Fenton Carcinogenesis 30 2009 Show Abstract Full Text Article | 19221001 | |
A conjugate of camptothecin and a somatostatin analog against prostate cancer cell invasion via a possible signaling pathway involving PI3K/Akt, alphaVbeta3/alphaVbeta5 and MMP-2/-9. Li-Chun Sun, Jing Luo, L Vienna Mackey, Joseph A Fuselier, David H Coy Cancer letters 246 157-66 2007 Show Abstract | 16644105 | |
MCP-1 overexpressed in tuberous sclerosis lesions acts as a paracrine factor for tumor development. Li, Shaowei, et al. J. Exp. Med., 202: 617-24 (2005) 2005 Show Abstract | 16129702 | |
cAMP-response element-binding protein mediates tumor necrosis factor-alpha-induced vascular smooth muscle cell migration Ono, Hiroki, et al Arterioscler Thromb Vasc Biol, 24:1634-9 (2004) 2004 | 15242860 | |
Neuronally expressed stem cell factor induces neural stem cell migration to areas of brain injury. Sun, Lixin, et al. J. Clin. Invest., 113: 1364-74 (2004) 2004 Show Abstract | Immunohistochemistry (Tissue) | 15124028 |
Transmembrane motility assay of transiently transfected cells by fluorescent cell counting and luciferase measurement. Gildea, J J, et al. BioTechniques, 29: 81-6 (2000) 2000 Show Abstract | 10907081 |
Brochure
Title |
---|
Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression |
Cell Migration and Invasion: Choosing the Right Assay |
EpiGRO™, EndoGRO™ and FibroGRO™ Reagents |