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2060 ReBlot Western Blot Recycling Kit

2060
1 kit  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      WB
      Description
      Catalogue Number2060
      Brand Family Chemicon®
      Trade Name
      • ReBlot
      • Chemicon
      DescriptionReBlot Western Blot Recycling Kit
      OverviewWestern blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electro-phoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.

      Stripping and re-probing of Western blots offers several advantages:

      1) Conservation of samples that are expensive or available only in limited quantities,

      2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,

      3) Re-analysis of anomalous results and confirmation with the same or a different antibody,

      4) Correcting errors in incubation with the wrong antibody,

      5) Cost savings in reagents and time by reusing the same blot.

      While antigen and antibody-based immunoaffinity matrices, such as Sepharose™ conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting.

      The MILLIPORE Re-Blot Western Blot Recycling Kit contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.

      Advantages of the Re-Blot Western Blot Recycling Kit include:

      ·*No pungent-smelling b-mercaptoethanol is contained in the Antibody Stripping Solution.

      ·*Antibody stripping is done at room temperature. No heating of blots is required.

      ·*Blots can be stripped of antibodies in approximately 10 minutes at room temperature.

      ·*Reblocking of blots may be avoided in most instances.

      ·*Blots may be reused in less than 15 minutes.
      Materials Required but Not Delivered· Standard Blot or blot strips, on nitrocellulose or PVDF/nylon membrane.

      · Alternative Blocking Solutions, such as 10% BSA, for antibodies that are not compatible with milk-based blotting agents.

      · Plastic Wrap, such as Saran Wrap, for storage of blots that will not be re-probed immediately.

      · Distilled Water, for reagent dilution.

      · Plastic Trays for incubation of blots or blot strips in stripping, washing and blocking solutions.

      · Positive and Negative Stripping Controls. It is recommended that one new strip (not subjected to stripping solution) be included for comparison purposes.
      References
      Product Information
      Components
      • Antibody Stripping Solution (10x) - (1 container, 50 mL).
      • Blocking Buffer (20x)
      • milk-based - (1 container, 50 mL).
      Detection methodChemiluminescence
      Applications
      Key Applications
      • Western Blotting
      Application NotesThe MILLIPORE Re-Blot Western Blot Recycling Kit is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site.

      The Re-Blot Western Blot Recycling Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.

      This product is for research use only; not for diagnostic or in vivo use.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsKit components should be stored at 2-8° C upon arrival. Product is stable for 3 to 6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37° C before use.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      2060 04053252276859

      Documentation

      References

      Reference overviewPub Med ID
      Immunohistochemical and functional correlations of renal cyclooxygenase-2 in experimental diabetes
      Komers, R., et.al.
      J. Clin. Invest., 107:889-898 (2001)  2001

      11285308 11285308
      Key role for constitutive cyclooxygenase-2 of MDCK cells in basal signaling and response to released ATP.
      Ostrom, R S, et al.
      Am. J. Physiol., Cell Physiol., 281: C524-31 (2001)  2001

      Show Abstract
      11443051 11443051

      Data Sheet

      Title
      ReBlot Western Blot Recycling Kit - Data Sheet