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71189 RoboPop™ GST•Bind™ Purification Kit

High-throughput, milligram-scale purification of His•Tag® and GST•Tag™ fusion proteins

RoboPop™ GST•Bind™ Purification Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71189
  
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      Overview

      Replacement Information

      This product has been discontinued.





      The RoboPop™ GST•Bind™ Purification Kit is designed for high-throughput (HT), filtration-based purification of soluble GST•Tag™ fusion proteins directly from E. coli cultures. Like the corresponding RoboPop GST•Mag™ Kit (Grabski 2002), this kit features PopCulture® Reagent, rLysozyme™ Solution, and Benzonase® Nuclease for centrifuge-free cell lysis and extract preparation in one step. This kit is designed for larger-scale cultures (up to 5 ml) and includes GST•Bind Resin plus a filtration-based processing protocol. Whereas the magnetic-based GST•Mag Kits purify up to 50 µg target protein per 1 ml culture, the filtration-based kits purify up to 0.8 mg GST•Tag fusion protein per well.

      Bacterial culture, cell lysis, and resin binding steps are carried out in standard 24- well plates (not supplied), which accommodate a maximum volume of 5 ml per well. The reaction slurry is then transferred to a 96-well Filter Plate (included) and the washing and elution steps are carried out on a vacuum manifold. The Filter Plate is compatible with standard filter manifolds for manual sample processing, and the entire purification has been validated for robotic sample processing with the MultiPROBE® II liquid handling workstation from PerkinElmer Life Sciences and with the Genesis® Freedom™ workstation from Tecan. A 96-well Collection Plate (1-ml wells) with an air-tight aluminum foil sealer is provided for storage of the purified proteins.

      Description


      Catalogue Number71189 Brand Family Novagen®
      References
      ReferencesGrabski, A., et al. 2002. inNovations 14, 2.
      Product Information
      •75 mlPopCulture® Reagent
      •25 mlNi-NTA His•Bind Resin
      •125 ml4X Ni-NTA Bind Buffer
      •2 x 125 ml4X Ni-NTA Wash Buffer
      •50 ml4X Ni-NTA Elute Buffer
      •12-ml 96-well Filter Plate
      •11-ml 96-well Collection Plate
      with Sealer
      •300 KUrLysozyme™ Solution
      (Note: 1 KU = 1000 units)
      •1 mlrLysozyme Dilution Buffer
      •10,000 UBenzonase® Nuclease,
      Purity > 90%
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71189 0

      Documentation

      RoboPop™ GST•Bind™ Purification Kit Certificates of Analysis

      TitleLot Number
      71189

      References

      Reference overview
      Grabski, A., et al. 2002. inNovations 14, 2.