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03-0211-00 SMC® Human pTau231 High Sensitivity Immunoassay Kit

The SMC® Human pTau231 High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure pTau231 in Human Serum, K2EDTA Plasma, and Cerebrospinal Fluid samples.

03-0211-00
96 well assay  
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      Overview

      Replacement Information
      Description
      Catalogue Number03-0211-00
      Trade Name
      • SMC®
      DescriptionSMC® Human pTau231 High Sensitivity Immunoassay Kit
      OverviewThe SMC® Human pTau231 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
      Background InformationThe SMC® Human pTau231 High Sensitivity Immunoassay uses a quantitative fluorescent sandwich immunoassay technique to measure pTau231 in human serum, plasma, or CSF samples. A capture antibody specific for human pTau231 has been pre-coated onto paramagnetic microparticles (beads). The user pipettes beads, standards, and samples into uncoated microplate wells. During incubation, the pTau231 present in the sample binds to the capture antibody on the coated beads. Unbound molecules are washed away during the subsequent wash steps. Fluor-labeled detection antibody is added to each well and incubated. This detection antibody recognizes and binds to pTau231 that has been captured onto the beads, thus completing the sandwich. Elution buffer is added to dissociate the protein sandwich, releasing the fluor-labeled antibodies. The eluted antibodies are transferred to a SMCxPRO® 384-well plate. The plate is loaded into the SMCxPRO® System where the labeled molecules are detected and counted. The number of fluor-labeled detection antibodies counted is directly proportional to the amount of pTau231 present in the sample. The amount of pTau231 in unknown samples is interpolated from a standard curve.
      References
      Product Information
      Components
      • Assay Buffer;pTau231 Coated Beads;Standard Diluent;pTau231 Detection Antibody;pTau231 Standard;10X Wash Buffer;Buffer D;Elution Buffer B
      Precision, %
      • The assay variations of SMC® Human pTau231 Immunoassay kits were studied using fifteen normal serum and plasma run in triplicate by 3 different operators on 3 different days.;Mean intra-assay variation was ≤10%.;Mean inter-assay variation was ≤20%.
      Quality LevelMQ200
      Applications
      ApplicationThe SMC® Human pTau231 High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure pTau231 in Human Serum, K2EDTA Plasma, and Cerebrospinal Fluid samples.
      Key Applications
      • Single Molecule Counting (SMC®)
      Application NotesTau protein is primarily a structural protein in neurons of the central nervous. Found as at least six different isoforms, these proteins are thought to provide structure, enable signaling, and regulate transport. In disease states, Tau proteins can become hyperphosphorylated and create tauopathies in the form of neurofibrillary or gliofibrillary tangles commonly associated with Alzheimer’s or Parkinson’s disease. Tau protein phosphorylated at threonine-231 is referred to as Phospho-Tau231 (pTau231). pTau231 is thought to be a promising marker for Alzheimer’s disease as it can be detected in serum or plasma and is strongly associated with physiological brain changes.
      Biological Information
      Cross ReactivityCross-reactivity to the following analyte was tested with the following result:
      pTau181: Not detected
      pTau217: Not detected
      Accuracy
      • Five (5) samples each of serum, plasma, and CSF were spiked with three different concentrations of pTau231 standard. Spike Recovery Range: Serum 68 - 100%, Plasma 44 - 68%, CSF 42 - 125%; Spike Recovery Mean: Serum 81%, K2EDTA Plasma 57%, CSF 96%
      Physicochemical Information
      Sensitivity
      • 0.49-3.91pg/mL, refer to CoA for lot-specific LLOQ
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      03-0211-00 04065270972378

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