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SCC271 Simplicon® Human iPS Cell Line

Highly characterized integration-free human induced pluripotent stem cell (iPSC) line generated using Simplicon® RNA reprogramming technology.

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SCC271
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC271
      DescriptionSimplicon® Human iPS Cell Line
      Alternate Names
      • Human iPSC Line
      • Human Pluripotent Stem Cell Line
      Background InformationThe discovery that somatic cells could revert back to pluripotent like cells in 2006 by Shinya Yamanaka created an entirely new area of stem cell biology. Human iPS cells removed the ethical concerns associated with embryonic stem cells and allowed scientists to model human diseases that were previously impossible to model such as Alzheimer’s, Parkinson’s and Autism. Human induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). The ability to expand pluripotent cells in vitro and subject them to direct differentiation to produce specific cell types is crucial for the development of potential cell-based therapies and disease-in-a-dish cell models for research.

      Simplicon® RNA Reprogramming Technology is a next generation non-integrating reprogramming system that uses a single synthetic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells. The single RNA strand contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1 (OKSG) and enables extremely efficient reprogramming using a single transfection step without any viral intermediates or host genome integration. The Simplicon® human iPS cell line was generated by reprogramming human foreskin fibrobalsts (SCC058) using the Simplicon® RNA Reprogramming Kit (SCR550). The cell line is integration-free, highly characterized and maintains a pluripotent phenotype over multiple passages.

      Reference: Dowdy, SF, et al. Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 1;13(2):246-54.
      References
      Product Information
      Applications
      ApplicationHighly characterized integration-free human induced pluripotent stem cell (iPSC) line generated using Simplicon® RNA reprogramming technology.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • iPSC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥1X106 viable cells.
      • Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsSimplicon® human iPS cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC271 04054839752858