189730 Streptavidin

189730
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Overview

Replacement Information

Key Spec Table

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
189730-10MG
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      189730-1MG
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      Limited AvailabilityLimited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
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        Remaining : Will advise
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          Plastic ampoule 1 mg
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          Description
          OverviewA high-affinity biotin binding protein.
          Catalogue Number189730
          Brand Family Calbiochem®
          SynonymsAvidin, Streptomyces avidinii
          References
          ReferencesEberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
          Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
          Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
          Roth, J., et al. 1992. Histochemistry 98, 229.
          Sano, T., et al. 1992. Science 258, 120.
          Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
          Srivastava, P., et al. 1992. Cytometry 13, 711.
          Product Information
          CAS number9013-20-1
          Unit of DefinitionOne unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
          FormLyophilized solid
          Quality LevelMQ100
          Applications
          Biological Information
          Specific Activity≥10 units/mg protein
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Moisture Protect from moisture
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Streptavidin SDS

          Title

          Safety Data Sheet (SDS) 

          Streptavidin Certificates of Analysis

          TitleLot Number
          189730

          References

          Reference overview
          Eberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
          Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
          Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
          Roth, J., et al. 1992. Histochemistry 98, 229.
          Sano, T., et al. 1992. Science 258, 120.
          Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
          Srivastava, P., et al. 1992. Cytometry 13, 711.

          Brochure

          Title
          Bulk Product Guide
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-April-2008 RFH
          SynonymsAvidin, Streptomyces avidinii
          DescriptionA high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
          FormLyophilized solid
          CAS number9013-20-1
          Specific activity≥10 units/mg protein
          Unit definitionOne unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
          SolubilityReconstitute in H₂O or 1 mM NaHCO₃, pH 9.0 at twice the final concentration and then dilute with an equal volume of 2X buffer.
          Storage -20°C
          Protect from moisture
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.
          Toxicity Standard Handling
          ReferencesEberle, J. and Seibl, R. 1992. J. Virol. Methods 40, 347.
          Gudibande, S.R., et al. 1992. Mol. Cell. Probes. 6, 495.
          Nesbitt, S.A. and Horton, M.A. 1992. Anal. Biochem. 206, 267.
          Roth, J., et al. 1992. Histochemistry 98, 229.
          Sano, T., et al. 1992. Science 258, 120.
          Scouten, W.H. and Konecny, P. 1992. Anal. Biochem. 205, 313.
          Srivastava, P., et al. 1992. Cytometry 13, 711.