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OR04L Streptavidin, Alkaline Phosphatase Conjugate

Streptavidin, Alkaline Phosphatase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OR04L
  
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      Overview

      Replacement Information
      Description
      Catalogue NumberOR04L
      Brand Family Calbiochem®
      References
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (10-200 ng/ml)
      Immunoblotting (10-200 ng/ml)
      Immunohistochemistry (10-200 ng/ml)
      Application CommentsDirect conjugates of streptavidin are provided to be used in combination with biotinylated Calbiochem® monoclonal antibodies. The recommended concentration is 10-200 ng/ml. Conjugates should be titrated for optimal results in individual systems (this is particularly important for immunofluorescence).
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized conjugate with 1 ml sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4. Lyophilized conjugates should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Rehydrated conjugates can be stored at 4°C for up to 1 week with 0.1% azide added.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OR04L 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      ApplicationELISA (10-200 ng/ml)
      Immunoblotting (10-200 ng/ml)
      Immunohistochemistry (10-200 ng/ml)
      DescriptionStreptavidin conjugated to alkaline phosphatase.
      BackgroundUse of streptavidin/biotin combinations as a detection system provides a high degree of sensitivity and minimal nonspecific binding resulting from the high specificity and high affinity of the streptavidin/biotin reaction. The streptavidin/biotin detection system, when used in conjunction with direct fluorescent conjugates of second step reagents, allows for the ability to perform dual fluorescent analysis.
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsDirect conjugates of streptavidin are provided to be used in combination with biotinylated Calbiochem® monoclonal antibodies. The recommended concentration is 10-200 ng/ml. Conjugates should be titrated for optimal results in individual systems (this is particularly important for immunofluorescence).
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized conjugate with 1 ml sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4. Lyophilized conjugates should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Rehydrated conjugates can be stored at 4°C for up to 1 week with 0.1% azide added.
      Toxicity Standard Handling