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574798 SynaptoGreen™ Reagent

SynaptoGreen™ Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FM1-43, Frie Mao 1-43, N-(3-Triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridinium, 2Br

574798
  
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      149838-22-2C₃₀H₄₉Br₂N₃
      Description
      Overview

      This product has been discontinued.





      A lipophilic, non-toxic water-soluble styrylpyridinium green fluoroprobe that is useful for staining active recycling synaptic vesicles. Also useful for studying endocytosis and in identifying cell membrane boundaries. The compound is non-fluorescent in aqueous solution but becomes fluorescent when incorporated into the plasma membrane. Reversible, photostable material that does not diffuse out of internalized vesicles. Can also be used in combination with the SynaptoRed™ Reagent (Cat. No. 574799) to image plasma membranes independently in two colors.
      Catalogue Number574798
      Brand Family Calbiochem®
      SynonymsFM1-43, Frie Mao 1-43, N-(3-Triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridinium, 2Br
      References
      ReferencesVazquez-Martinez, R., et al. 2001. Endocrinology 142, 2095.
      Durand, J., et al. 2000. Neuroscience 97, 11.
      Cochilla, A.J., et al. 1999. Annu. Rev. Neurosci. 22, 1.
      Raucher, D. and Sheetz, M.P. 1999. J. Cell. Biol. 144, 497.
      Ryan, T.A., et al. 1997. Nature 388, 478.
      Henkel, A.W., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1918.
      Liu, G. and Tsein, R.W. 1995. Nature 375, 404.
      Betz, W.J. and Bewick, G.S. 1992. Science 255, 200.
      Product Information
      CAS number149838-22-2
      FormRed solid
      Hill FormulaC₃₀H₄₉Br₂N₃
      Chemical formulaC₃₀H₄₉Br₂N₃
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      574798 0

      Documentation

      SynaptoGreen™ Reagent Certificates of Analysis

      TitleLot Number
      574798

      References

      Reference overview
      Vazquez-Martinez, R., et al. 2001. Endocrinology 142, 2095.
      Durand, J., et al. 2000. Neuroscience 97, 11.
      Cochilla, A.J., et al. 1999. Annu. Rev. Neurosci. 22, 1.
      Raucher, D. and Sheetz, M.P. 1999. J. Cell. Biol. 144, 497.
      Ryan, T.A., et al. 1997. Nature 388, 478.
      Henkel, A.W., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1918.
      Liu, G. and Tsein, R.W. 1995. Nature 375, 404.
      Betz, W.J. and Bewick, G.S. 1992. Science 255, 200.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-September-2008 RFH
      SynonymsFM1-43, Frie Mao 1-43, N-(3-Triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridinium, 2Br
      DescriptionA lipophilic, nontoxic, water-soluble styrylpyridinium green fluoroprobe that is useful for staining active recycling synaptic vesicles. Also useful for studying endocytosis, membrane turnover, and identifying cell membrane boundaries. This compound is non-fluorescent in aqueous solution, but becomes fluorescent when incorporated into the plasma membrane. Reversible, photostable material that does not diffuse out of internalized vesicles. Can also be used in combination with the SynaptoRed™ (Cat. No. 574799) to image plasma membranes independently in two colors. Excitation max.: ~510; emission max.: ~625 nm.
      FormRed solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number149838-22-2
      Chemical formulaC₃₀H₄₉Br₂N₃
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml), H₂O (2 mg/ml), or methanol (5 mg/ml)
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesVazquez-Martinez, R., et al. 2001. Endocrinology 142, 2095.
      Durand, J., et al. 2000. Neuroscience 97, 11.
      Cochilla, A.J., et al. 1999. Annu. Rev. Neurosci. 22, 1.
      Raucher, D. and Sheetz, M.P. 1999. J. Cell. Biol. 144, 497.
      Ryan, T.A., et al. 1997. Nature 388, 478.
      Henkel, A.W., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 1918.
      Liu, G. and Tsein, R.W. 1995. Nature 375, 404.
      Betz, W.J. and Bewick, G.S. 1992. Science 255, 200.