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616402 TACE Substrate II, Fluorogenic

TACE Substrate II, Fluorogenic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TNF-α Convertase Enzyme Substrate, Fluorogenic, MCA-PLAQAV(Dpa)RSSSR-NH₂, ADAM-17 Substrate II, Fluorogenic

616402
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      A fluorogenic peptide substrate for TACE (Km = ~ 19 µM). Substrate cleavage occurs at the Ala-Val bond. Useful for screening TACE Inhibitors.
      Catalogue Number616402
      Brand Family Calbiochem®
      SynonymsTNF-α Convertase Enzyme Substrate, Fluorogenic, MCA-PLAQAV(Dpa)RSSSR-NH₂, ADAM-17 Substrate II, Fluorogenic
      References
      ReferencesPatel, I.R., et al. 1998. J. Immunol. 160, 4570.
      Van Dyk, D.E., et al. 1997. Bioorg. Med. Chem. Lett. 7, 1219.
      Product Information
      FormLiquid
      FormulationA 4 mM (7.5 mg/ml) solution in DMSO.
      Hill FormulaC₆₉H₁₀₃N₂₃O₂₄
      Chemical formulaC₆₉H₁₀₃N₂₃O₂₄
      Applications
      Biological Information
      Purity≥90% by HPLC
      Physicochemical Information
      Peptide SequenceMCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂[Dpa = N-3-(2,4-Dinitrophenyl)-L-2,3-diaminopropionyl]
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      616402 0

      Documentation

      References

      Reference overview
      Patel, I.R., et al. 1998. J. Immunol. 160, 4570.
      Van Dyk, D.E., et al. 1997. Bioorg. Med. Chem. Lett. 7, 1219.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-September-2008 RFH
      SynonymsTNF-α Convertase Enzyme Substrate, Fluorogenic, MCA-PLAQAV(Dpa)RSSSR-NH₂, ADAM-17 Substrate II, Fluorogenic
      DescriptionA fluorescence energy transfer (FRET) peptide substrate for tumor necrosis factor (TNF)-α convertase (TACE) (Km ~19 µM). Cleavage of this TACE substrate II occurs between the alanine and valine residues. Useful for the screening of TACE inhibitors. Excitation max: ~320 nm; emission max: ~395 nm.
      FormLiquid
      FormulationA 4 mM (7.5 mg/ml) solution in DMSO.
      Recommended reaction conditions50 mM Tricine buffer, pH 7.5, containing 100 mM NaCl, 10 mM CaCl2, and 1 mM ZnCl2, 10 µM TACE Substrate II, 600 nM TACE; incubate at 27°C with mechanical shaking; reaction can be quenched with 50 mM EDTA (final concentration); cleavage monitored at excitation at ~320 nm and emission at ~395 nm.
      Chemical formulaC₆₉H₁₀₃N₂₃O₂₄
      Peptide SequenceMCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂[Dpa = N-3-(2,4-Dinitrophenyl)-L-2,3-diaminopropionyl]
      Purity≥90% by HPLC
      Storage Protect from light
      Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesPatel, I.R., et al. 1998. J. Immunol. 160, 4570.
      Van Dyk, D.E., et al. 1997. Bioorg. Med. Chem. Lett. 7, 1219.