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614852 Topoisomerase IIα Inhibitor, TSC24 - Calbiochem

The Topoisomerase IIα Inhibitor, TSC24 controls the biological activity of Topoisomerase. This small molecule/inhibitor is primarily used for Cell Structure applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (E)-N,N-dimethyl-2-(quinolin-2-ylmethylene)hydrazinecarbothioamide

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614852
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₃H₁₄N₄S

Pricing & Availability

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614852-10MG
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      Description
      OverviewA cell-permeable, TSC (thiosemicarbazone) derivative that acts as a selective and ATP competitive Topoisomerase IIα inhibitor and Fe chelator. Independent of its Fe chelating ability, this compound is shown to block Topo II&alpha-catalyzed ATP hydrolysis by competing with ATP for binding to the ATPase domain of Topo IIα (Kd = 18 µM). It also displays M phase cell-cycle arrest at 1 µM in nocodazole-treated HT-29 cells. This compound demonstrates anti-proliferative activity, without distinct or obvious selectivity, towards HeLa, HL-60, SGC7901, and HT-29 cultures (IC50 = 90 nM, 50 nM, 30 nM, and 20 nM, respectively), and a broader panel of tumor cell lines with IC50 values in the nanomolar range.
      Catalogue Number614852
      Brand Family Calbiochem®
      Synonyms(E)-N,N-dimethyl-2-(quinolin-2-ylmethylene)hydrazinecarbothioamide
      References
      ReferencesHuang, H., et al. 2010. J. Med. Chem. 53, 3048.
      Product Information
      FormYellow powder
      Hill FormulaC₁₃H₁₄N₄S
      Chemical formulaC₁₃H₁₄N₄S
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      614852-10MG 04055977186352

      Documentation

      Topoisomerase IIα Inhibitor, TSC24 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Topoisomerase IIα Inhibitor, TSC24 - Calbiochem Certificates of Analysis

      TitleLot Number
      614852

      References

      Reference overview
      Huang, H., et al. 2010. J. Med. Chem. 53, 3048.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-February-2011 RFH
      Synonyms(E)-N,N-dimethyl-2-(quinolin-2-ylmethylene)hydrazinecarbothioamide
      DescriptionA cell-permeable, TSC (thiosemicarbazone) derivative that acts as a selective and ATP competitive Topoisomerase IIα inhibitor and Fe chelator. Independent of its Fe chelating ability, this compound is shown to block Topo II&alpha-catalyzed ATP hydrolysis by competing with ATP for binding to the ATPase domain of Topo IIα (Kd = 18 µM). It also displays M phase cell-cycle arrest at 1 µM in nocodazole-treated HT-29 cells. This compound demonstrates anti-proliferative activity, without distinct or obvious selectivity, towards HeLa, HL-60, SGC7901, and HT-29 cultures (IC50 = 90 nM, 50 nM, 30 nM, and 20 nM, respectively), and a broader panel of tumor cell lines with IC50 values in the nanomolar range.
      FormYellow powder
      Chemical formulaC₁₃H₁₄N₄S
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (15 mg/ml)
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesHuang, H., et al. 2010. J. Med. Chem. 53, 3048.