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654161 Tubulin-α-1, His•Tag® Fusion, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Tubulin-α-1, Human, Recombinant

654161
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human tubulin-α-1 (TBA1, TUBA1, α-tubulin 1) fused to His•Tag® and S•Tag™ sequences at the N-terminus and expressed in E. coli. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Tubulin-α-1 is a major constituent of microtubules. It binds one GTP at a nonexchangeable site (aa 142-148) on the alpha-chain. IFN-α-induced phosphorylation at Tyr271 has been demonstrated in Jurkat cells. Tyrosine phosphorylation is expected at Tyr172, Tyr210, Tyr224, and Tyr282 by EGFR and at Tyr440 by InsR.
      Catalogue Number654161
      Brand Family Calbiochem®
      SynonymsTubulin-α-1, Human, Recombinant
      Application Data
      References
      ReferencesLennon, G., et al. 1996. Genomics 33, 151.
      Cox, M.E. and Maness, P.F. 1993. J. Mol. Neurosci. 4, 63.
      Matten, W.T., et al. 1990. J. Cell. Biol. 111, 1959.
      Product Information
      FormLiquid
      FormulationIn PBS, 6 M Urea, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138).
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      654161 0

      Documentation

      Tubulin-α-1, His•Tag® Fusion, E. coli Certificates of Analysis

      TitleLot Number
      654161

      References

      Reference overview
      Lennon, G., et al. 1996. Genomics 33, 151.
      Cox, M.E. and Maness, P.F. 1993. J. Mol. Neurosci. 4, 63.
      Matten, W.T., et al. 1990. J. Cell. Biol. 111, 1959.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-September-2008 RFH
      SynonymsTubulin-α-1, Human, Recombinant
      Application Data
      DescriptionFull-length, recombinant, human tubulin-α-1 (TBA1, TUBA1, α-tubulin 1) fused to His•Tag® and S•Tag™ sequences at the N-terminus and expressed in E. coli. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Tubulin-α-1 is a major constituent of microtubules. It binds one GTP at a nonexchangeable site (aa 142-148) on the alpha-chain. IFN-α-induced phosphorylation at Tyr271 has been demonstrated in Jurkat cells. Tyrosine phosphorylation is expected at Tyr172, Tyr210, Tyr224, and Tyr282 by EGFR and at Tyr440 by InsR.
      FormLiquid
      FormulationIn PBS, 6 M Urea, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138).
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Irritant
      ReferencesLennon, G., et al. 1996. Genomics 33, 151.
      Cox, M.E. and Maness, P.F. 1993. J. Mol. Neurosci. 4, 63.
      Matten, W.T., et al. 1990. J. Cell. Biol. 111, 1959.