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662053 UbcH3 Conjugating Enzyme E2, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CDC34, UBE2R2

662053
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human UbcH3 fused at the N-terminus to His•Tag® sequence and expressed in E. coli. UbcH3, an E2 ubiquitin conjugase homologous to Cdc34 in yeast, is important in the control of cell cycle and DNA replication. UbcH3/Cdc34 in association with different E3 complexes, including SCF, has been shown to target proteins including IκB, B-Myb, Wee1, MyoD, ICERIIγ, ATF5, p27Xic1, and p27Kip1. Other targets such as β-catenin, p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of Cdc34 by virtue of their SCF requirement for proteolysis. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      Catalogue Number662053
      Brand Family Calbiochem®
      SynonymsCDC34, UBE2R2
      References
      ReferencesPintard, L., et al. 2003. Nat. Cell. Biol. 5, 856.
      Varelas, X., et al. 2003. Mol. Cell. Biol. 23, 5388.
      Gonen, H., et al. 1999. J. Biol. Chem. 274, 14823.
      Seol, J.H., et al. 1999. Gene. Dev. 13, 1614.
      Listwan, J., et al. 1998. EMBO. J. 17, 368.
      King, R.W., et al. 1996. Science 274, 1652.
      Ptak, C., et al. 1994. J. Biol. Chem. 269, 26539.
      Plon, S.E., et al. 1993. Proc. Natl. Acad. Sci. 90, 10484.
      Goebl, M.G., et al. 1988. Science 241, 1331.
      Product Information
      FormulationIn 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 8.0.
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      662053 0

      Documentation

      UbcH3 Conjugating Enzyme E2, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      662053

      References

      Reference overview
      Pintard, L., et al. 2003. Nat. Cell. Biol. 5, 856.
      Varelas, X., et al. 2003. Mol. Cell. Biol. 23, 5388.
      Gonen, H., et al. 1999. J. Biol. Chem. 274, 14823.
      Seol, J.H., et al. 1999. Gene. Dev. 13, 1614.
      Listwan, J., et al. 1998. EMBO. J. 17, 368.
      King, R.W., et al. 1996. Science 274, 1652.
      Ptak, C., et al. 1994. J. Biol. Chem. 269, 26539.
      Plon, S.E., et al. 1993. Proc. Natl. Acad. Sci. 90, 10484.
      Goebl, M.G., et al. 1988. Science 241, 1331.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2009 JSW
      SynonymsCDC34, UBE2R2
      DescriptionFull-length, recombinant, human UbcH3 fused at the N-terminus to His•Tag® sequence and expressed in E. coli. UbcH3, an E2 ubiquitin conjugase homologous to Cdc34 in yeast, is important in the control of cell cycle and DNA replication. UbcH3/Cdc34 in association with different E3 complexes, including SCF, has been shown to target proteins including IκB, B-Myb, Wee1, MyoD, ICERIIγ, ATF5, p27Xic1, and p27Kip1. Other targets such as β-catenin, p21Cip1, E2F, cyclin E, and cyclin D are putative substrates of Cdc34 by virtue of their SCF requirement for proteolysis. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      FormulationIn 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Typical enzyme concentration required for in vitro conjugation is 100 nM to 1 µM
      Purity≥95% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesPintard, L., et al. 2003. Nat. Cell. Biol. 5, 856.
      Varelas, X., et al. 2003. Mol. Cell. Biol. 23, 5388.
      Gonen, H., et al. 1999. J. Biol. Chem. 274, 14823.
      Seol, J.H., et al. 1999. Gene. Dev. 13, 1614.
      Listwan, J., et al. 1998. EMBO. J. 17, 368.
      King, R.W., et al. 1996. Science 274, 1652.
      Ptak, C., et al. 1994. J. Biol. Chem. 269, 26539.
      Plon, S.E., et al. 1993. Proc. Natl. Acad. Sci. 90, 10484.
      Goebl, M.G., et al. 1988. Science 241, 1331.