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15-105 mTOR phosphorylation Pathway Explorer Antibody MiniPack

This Antibody pack of Anti-mTOR antibodies contains Anti-phospho-mTOR Antibody(Ser2481), Anti-phospho-mTOR Antibody (Thr2446), Anti-phospho-mTOR (Ser2448).

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Research Category: Signaling, Apoptosis & Cancer Research Sub-Category: PI3K, Akt, & mTOR Signaling, Glucose/Glycogen Metabolism, Insulin/Energy Signaling Gene Symbol: FRAP1, RAPT1, mTOR, FRAP, MTOR, FLJ44809, FRAP2, RAFT1, FRAP1 FK506 binding protein 12-rapamycin associated protein 1, mammalian target of rapamycin UniProt Number: P42345
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15-105
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      Description
      Catalogue Number15-105
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionmTOR phosphorylation Pathway Explorer Antibody MiniPack
      Alternate Names
      • FKBP12-rapamycin complex-associated protein 1
      • FKBP-rapamycin associated protein
      • Mammalian target of rapamycin
      • Rapamycin target protein
      • rapamycin associated protein FRAP2
      Background InformationPathway Explorer Antibody MiniPack:
      Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).

      mTOR:
      mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

      *See full size versions for corresponding references.
      References
      Product Information
      Components
      • 09-343SP Anti-phospho-mTOR (Ser2481); 30 µL
      • 09-345SP Anti-phospho-mTOR (Thr2446); 30 µL
      • 09-213SP Anti-phospho-mTOR (Ser2448); 60 µL
      FormatAffinity Purified
      Presentation3 individual tubes each containing antibodies directed against one of the three phosphorylated forms of mTOR (Thr2446, Ser2448, and Ser2481)
      PropertiesEach vial is 30% the size of the parent catalog number
      Quality LevelMQ100
      Applications
      ApplicationThis Antibody pack of Anti-mTOR antibodies contains Anti-phospho-mTOR Antibody(Ser2481), Anti-phospho-mTOR Antibody (Thr2446), Anti-phospho-mTOR (Ser2448).
      Application NotesPathway Explorer Antibody MiniPack
      Biological Information
      Epitope09-345SP:phosphorylated Thr2446 of mTOR
      09-213SP: phosphorylated Ser2448 of mTOR
      09-343SP: phosphorylated Ser2481 of mTOR
      HostRabbit
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
      Gene Symbol
      • FRAP1
      • RAPT1
      • mTOR
      • FRAP
      • MTOR
      • FLJ44809
      • FRAP2
      • RAFT1
      • FRAP1 FK506 binding protein 12-rapamycin associated protein 1
      • mammalian target of rapamycin
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
      SIZE: 2549 amino acids; 288892 Da
      SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
      TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
      SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size3/vials
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      15-105 04053252006357