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70225 pBACgus-6 DNA

pBACgus-6 DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70225
  
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      This product has been discontinued.





      pBACgus-6 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection. A modified gp64 tandem promoter contains both an immediate early promoter for expression beginning immediately after infection and a late promoter for continued expression in the late phase of infection. The pBACgus-6 Transfer Plasmid enables isolation of stably-transfected insect cell lines expressing target proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the gp64 promoter is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.
      Catalogue Number70225
      Brand Family Novagen®
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      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Catalogue Number GTIN
      70225 0

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits