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69419 pET-3b DNA - Novagen

69419
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69419-3
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-3a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors; the pET-23a-d(+) series corresponds to pET-3a-d but incorporates several additional features. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB026).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69419
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pET-3b DNA - Novagen Certificates of Analysis

      TitleLot Number
      69419

      Citations

      Title
    • Taj Kattapuram, et al. (2005) Protein kinase CK1α regulates mRNA binding by hnRNP-C in response to physiologic levels of hydrogen peroxide. Journal of Biological Chemistry 280, 15340-15347.
    • Takeki Ogata, et al. (2005) Betacellulin-δ4, a novel differentiation factor for pancreatic β-cells, ameliorates glucose intolerance in streptozotocin-treated rats. Endocrinology 146, 4673-4681.
    • Michio Yasunami, et al. (1995) Molecular characterization of cDNA encoding a novel protein related to transcriptional enhancer factor-1 from neural precursor cells. Journal of Biological Chemistry 270, 18649-18654.
    • P. Neubauer and K. Hofmann. (1994) Efficient use of lactose for the lac promoter-controlled overexpression of the main antigenic protein of the foot and mouth disease virus in Escherichia coli under fed-batch fermentation conditions. FEMS Microbiology Reviews 14, 99-102.
    • P. Neubauer, et al. (1992) Maximizing the expression of a recombinant gene in Escherichia coli by manipulation of induction time using lactose as an inducer. Applied Microbiology and Biotechnology 36, 739-744.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB026VM pET-3a-d Vector Map

      Vector Sequence

      Title
      pET-3b Vector Sequence

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors