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71464 pET-50b(+) DNA - Novagen

71464
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-50b(+) vector is designed for cloning and high-level expression of target proteins fused with the 495 aa NusA (Nus•Tag™) protein that is cleavable with the human rhinovirus (HRV) 3C protease (1). The NusA protein has been identified as having the highest potential for solubility when a data base of more than 4000 E. coli proteins was subjected to solubility modeling (2,3). Greater than 85% of the expressed protein was soluble in tests with each of four different NusA fusion proteins (2). Cloning sites are also available for producing fusion proteins containing two cleavable N-terminal His•Tag® sequences for detection and purification. The additional N-terminal His•Tag sequence significantly enhances purification yields when utilizing His•Mag™ Agarose Beads for high throughput purification (4). The plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the HRV 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that encodes restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (5).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71464
      Brand Family Novagen®
      References
      References1. Walker, P.A., Leong L. E.-C., Ng, P.W.P., Tan, S.H.,Waller, S., Murphy, D. and Porter, A.G. (1994) Bio/Technology 12, 601-605.

      2. Harrison, R.G. (2000) inNovations 11, 4-7.

      3. Davis, G.D., Elisee, C., Newham, D.M. and Harrison, R.G. (1998) Biotechnol. Bioeng. 65, 382-388.

      4. Novy, R. and Drott, D. (2002) inNovations 14, 12-13.

      5. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348-356.
      Product Information
      Fusion tagHis•Tag, Nus•Tag, S•Tag
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pET-50b(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      71464

      References

      Reference overview
      1. Walker, P.A., Leong L. E.-C., Ng, P.W.P., Tan, S.H.,Waller, S., Murphy, D. and Porter, A.G. (1994) Bio/Technology 12, 601-605.

      2. Harrison, R.G. (2000) inNovations 11, 4-7.

      3. Davis, G.D., Elisee, C., Newham, D.M. and Harrison, R.G. (1998) Biotechnol. Bioeng. 65, 382-388.

      4. Novy, R. and Drott, D. (2002) inNovations 14, 12-13.

      5. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348-356.

      Brochure

      Title
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

      User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB418VM pET-50b(+) Vector Map