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70783 pET Expression System 31b - Novagen

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      Replacement Information

      This product has been discontinued.

      pET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 31b
      The pET-31b(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein (1). The unique AlwN I cloning site allows the unidirectional insertion of of tandemly repeated peptide coding regions separated by methionine codons. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB098VM.

      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

      Catalogue Number70783
      Brand Family Novagen®
      References1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.
      Product Information
      10 µgpET-31b(+) vector DNA
      0.2 mlHost bacterial strain BLR(DE3)pLysS, glycerol stock
      0.2 mlInduction control clone, glycerol stock
      Fusion tagHis•Tag
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalogue Number GTIN
      70783 0


      pET Expression System 31b - Novagen Certificates of Analysis

      TitleLot Number


      Reference overview
      1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.


    • Graeme S. Garvey, Susan P. McCormick and Ivan Rayment. (2008) Structural and functional characterization of the TRI101 trichothecene 3-O-acetyltransferase from Fusarium sporotrichioides and Fusarium graminearum: kinetic insights to combating Fusarium head blight. Journal of Biological Chemistry 283, 1660-1669.
    • Min-Ju Kim, et al. (2007) NMR structural studies of interactions of a small, nonpeptidyl Tpo mimic with the thrombopoietin receptor extracellular juxtamembrane and transmembrane domains. Journal of Biological Chemistry 282, 14253-14261.
    • James B. Ames, et al. (2006) Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin. Journal of Biological Chemistry 281, 37237-37245.
    • Ana Beloqui, et al. (2006) Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships. Journal of Biological Chemistry 281, 22933-22942.
    • Jean-Louis Baneres, et al. (2005) Molecular characterization of a purified 5-HT4 receptor. A structural basis for drug efficacy. Journal of Biological Chemistry 280, 20253-20260.
    • Khang Thai, et al. (2005) Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins. Protein Science 14, 948-955.
    • Juan Carlos Rodriguez, Lilly Wong and Patricia A. Jennings. (2003) The solvent in CNBr cleavage reactions determines the fragmentation efficiency of ketosteroid isomerase fusion proteins used in the production of recombinant peptides. Protein Expression and Purification 28, 224-231.
    • User Protocols

      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual
      TB104 pET Peptide Expression System 31

      Vector Map

      TB098VM pET-31b(+) Vector Map