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71328 pET Expression System 45b

pET vectors plus host strains with induction control

pET Expression System 45b MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71328
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      pET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 45b
      The pET Expression System 4b5 contains 10 µg of pET-45b(+) DNA. pET-45b carries an N-terminal His•Tag® coding sequence that is cleavable with enterokinase. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB396.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      Catalogue Number71328
      Brand Family Novagen®
      References
      Product Information
      •10 µgpET Vector DNA
      •0.2 mlHost bacterial strains BL21, BL21(DE3) and BL21(DE3) pLysS Glycerol Stocks
      •0.2 mlInduction Control
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71328 0

      Documentation

      pET Expression System 45b Certificates of Analysis

      TitleLot Number
      71328

      Citations

      Title
    • Robert T. Glover, et al. (2007) The two-component regulatory system senX3-regX3 regulates phosphate-dependent gene expression in Mycobacterium smegmatis. Journal of Bacteriology 189, 5495-5503.
    • Guifeng Sun, et al. (2007) Structural and functional analyses of the major outer membrane protein of Chlamydia trachomatis. Journal of Bacteriology 189, 6222-6235.
    • Jun Dai, et al. (2005) The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment. Genes and Development 19, 472-488.
      		•

      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB396VM pET-45b(+) Vector Map