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71364 pRSF-2 Ek/LIC Vector Kit - Novagen

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      This product has been discontinued.

      The pRSF-2 Ek/LIC Vector is a LIC-compatible derivative of pRSF-1b. The plasmid contains a strong T7lac promoter and an amino-terminal His•Tag® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pCDF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.

      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71364
      Brand Family Novagen®
      References1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
      Product Information
      1 µgEk/LIC Vector
      25 unitsT4 DNA Polymerase, LIC-qualified
      50 µl25 mM EDTA
      40 µl25 mM dATP
      8 µlEk/LIC β-gal Control Insert
      100 µl100 mM DTT
      50 µl10X T4 DNA Polymerase Buffer
      1.5 mlNuclease-free Water
      5 × 2 mlSOC Medium
      10 µlTest Plasmid
      0.2 mlBL21(DE3)pLysS Competent Cells
      0.2 mlBL21(DE3) Competent Cells
      22 × 50 µlNovaBlue GigaSingles™ Competent Cells
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalogue Number GTIN
      71364 0


      pRSF-2 Ek/LIC Vector Kit - Novagen Certificates of Analysis

      TitleLot Number


      Reference overview
      1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.


      Radiance! Multisystem Protein Expression

      User Protocols

      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB163 Ek/LIC Cloning Kits
      TB401 pRSF and pCDF Vectors

      Vector Map

      TB395VM pRSF-2 Ek/LIC Vector Map

      Vector Sequence

      pRSF-2 Ek/LIC Vector Sequence