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70186 pT7Blue-2 Blunt Vector (linearized) - Novagen

70186
  
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      Overview

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      Overview

      This product has been discontinued.





      The pT7Blue-2 vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), f1 origin of replication and modified multiple cloning region designed for optimal expression of cloned inserts by in vitro translation. The multiple cloning region contains a T7 promoter upstream of the Xenopus β-globin 5′ UTR translation enhancer and S•Tag™ sequences, followed by an enterokinase cleavage site and extensive region of cloning sites. An SP6 promoter is placed within the multiple cloning sites to allow the synthesis of essentially unfused RNA from DNA inserted downstream.

      Catalogue Number70186
      Brand Family Novagen®
      References
      References

      Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

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      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Documentation

      pT7Blue-2 Blunt Vector (linearized) - Novagen Certificates of Analysis

      TitleLot Number
      70186

      References

      Reference overview

      Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB183 Perfectly Blunt® Cloning Kits

      Vector Sequence

      Title
      pT7Blue-2 Sequence

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      Categories

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors