Millipore Sigma Vibrant Logo
 

07-227


73 Results Advanced Search  
Showing
Products (0)
Documents (49)
Site Content (24)

Narrow Your Results Use the filters below to refine your search

Document Type

  • (27)
  • (20)
  • (2)
Can't Find What You're Looking For?
Contact Customer Service

 

  • Coronin-1A links cytoskeleton dynamics to TCR alpha beta-induced cell signaling. 18941544

    Actin polymerization plays a critical role in activated T lymphocytes both in regulating T cell receptor (TCR)-induced immunological synapse (IS) formation and signaling. Using gene targeting, we demonstrate that the hematopoietic specific, actin- and Arp2/3 complex-binding protein coronin-1A contributes to both processes. Coronin-1A-deficient mice specifically showed alterations in terminal development and the survival of alpha beta T cells, together with defects in cell activation and cytokine production following TCR triggering. The mutant T cells further displayed excessive accumulation yet reduced dynamics of F-actin and the WASP-Arp2/3 machinery at the IS, correlating with extended cell-cell contact. Cell signaling was also affected with the basal activation of the stress kinases sAPK/JNK1/2; and deficits in TCR-induced Ca2+ influx and phosphorylation and degradation of the inhibitor of NF-kappaB (I kappa B). Coronin-1A therefore links cytoskeleton plasticity with the functioning of discrete TCR signaling components. This function may be required to adjust TCR responses to selecting ligands accounting in part for the homeostasis defect that impacts alpha beta T cells in coronin-1A deficient mice, with the exclusion of other lympho/hematopoietic lineages.
    Document Type:
    Reference
    Product Catalog Number:
    07-227

  • Actin assembly controlled by GDP-Rab27a is essential for endocytosis of the insulin secretory membrane. 20138020

    We have recently reported that GDP-bound Rab27a regulates endocytosis of the insulin secretory membrane via its binding to coronin 3, an actin-binding protein. The aim of this study was to examine the participation of actin assembly in the Rab27a-dependent regulation of endocytosis using a pancreatic beta cell line, MIN6. Coronin 3 promoted F-actin bundling only in the presence of GDP-Rab27a. This effect was independent of coronin-3-binding to the actin-related proteins 2 and 3 (Arp2/3). Uptake of anti-phogrin-lumen antibody into MIN6 was inhibited by anti-coronin-3-C antibody which recognizes the actin-binding site. This inhibition was also observed with coronin-3-R28D, which lacks in actin binding. These results suggest that coronin 3 is a genuine GDP-Rab27a effector, and that controls endocytosis of the secretory membrane via modulating actin assembly in pancreatic beta-cells.
    Document Type:
    Reference
    Product Catalog Number:
    07-227

  • Phosphorylation of CRN2 by CK2 regulates F-actin and Arp2/3 interaction and inhibits cell migration. 22355754

    CRN2 (synonyms: coronin 1C, coronin 3) functions in the re-organization of the actin network and is implicated in cellular processes like protrusion formation, secretion, migration and invasion. We demonstrate that CRN2 is a binding partner and substrate of protein kinase CK2, which phosphorylates CRN2 at S463 in its C-terminal coiled coil domain. Phosphomimetic S463D CRN2 loses the wild-type CRN2 ability to inhibit actin polymerization, to bundle F-actin, and to bind to the Arp2/3 complex. As a consequence, S463D mutant CRN2 changes the morphology of the F-actin network in the front of lamellipodia. Our data imply that CK2-dependent phosphorylation of CRN2 is involved in the modulation of the local morphology of complex actin structures and thereby inhibits cell migration.
    Document Type:
    Reference
    Product Catalog Number:
    07-227

  • Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation. 19704022

    Invadopodia are matrix-degrading membrane protrusions in invasive carcinoma cells. The mechanisms regulating invadopodium assembly and maturation are not understood. We have dissected the stages of invadopodium assembly and maturation and show that invadopodia use cortactin phosphorylation as a master switch during these processes. In particular, cortactin phosphorylation was found to regulate cofilin and Arp2/3 complex-dependent actin polymerization. Cortactin directly binds cofilin and inhibits its severing activity. Cortactin phosphorylation is required to release this inhibition so cofilin can sever actin filaments to create barbed ends at invadopodia to support Arp2/3-dependent actin polymerization. After barbed end formation, cortactin is dephosphorylated, which blocks cofilin severing activity thereby stabilizing invadopodia. These findings identify novel mechanisms for actin polymerization in the invadopodia of metastatic carcinoma cells and define four distinct stages of invadopodium assembly and maturation consisting of invadopodium precursor formation, actin polymerization, stabilization, and matrix degradation.
    Document Type:
    Reference
    Product Catalog Number:
    07-227

  • Glia maturation factor gamma regulates the migration and adherence of human T lymphocytes. 22510515

    Lymphocyte migration and chemotaxis are essential for effective immune surveillance. A critical aspect of migration is cell polarization and the extension of pseudopodia in the direction of movement. However, our knowledge of the underlying molecular mechanisms responsible for these events is incomplete. Proteomic analysis of the isolated leading edges of CXCL12 stimulated human T cell lines was used to identify glia maturation factor gamma (GMFG) as a component of the pseudopodia. This protein is predominantly expressed in hematopoietic cells and it has been shown to regulate cytoskeletal branching. The present studies were undertaken to examine the role of GMFG in lymphocyte migration.Microscopic analysis of migrating T-cells demonstrated that GMFG was distributed along the axis of movement with enrichment in the leading edge and behind the nucleus of these cells. Inhibition of GMFG expression in T cell lines and IL-2 dependent human peripheral blood T cells with shRNAmir reduced cellular basal and chemokine induced migration responses. The failure of the cells with reduced GMFG to migrate was associated with an apparent inability to detach from the substrates that they were moving on. It was also noted that these cells had an increased adherence to extracellular matrix proteins such as fibronectin. These changes in adherence were associated with altered patterns of β1 integrin expression and increased levels of activated integrins as detected with the activation specific antibody HUTS4. GMFG loss was also shown to increase the expression of the β2 integrin LFA-1 and to increase the adhesion of these cells to ICAM-1.The present studies demonstrate that GMFG is a component of human T cell pseudopodia required for migration. The reduction in migration and increased adherence properties associated with inhibition of GMFG expression suggest that GMFG activity influences the regulation of integrin mediated adhesion.
    Document Type:
    Reference
    Product Catalog Number:
    Multiple
    Product Catalog Name:
    Multiple

  • Anti-p34-Arc/ARPC2 - 3714336

    Document Type:
    Certificate of Analysis
    Lot Number:
    3714336
    Product Catalog Number:
    07-227-I-25UG
    Product Catalog Name:
    Anti-p34-Arc/ARPC2

  • Anti-p34-Arc/ARPC2 - 4209919

    Document Type:
    Certificate of Analysis
    Lot Number:
    4209919
    Product Catalog Number:
    07-227-I-100UG
    Product Catalog Name:
    Anti-p34-Arc/ARPC2

  • Anti-p34-Arc/ARPC2 - 4120693

    Document Type:
    Certificate of Analysis
    Lot Number:
    4120693
    Product Catalog Number:
    07-227-I-100UG
    Product Catalog Name:
    Anti-p34-Arc/ARPC2

  • Anti-p34-Arc/ARPC2 - Q3244113

    Document Type:
    Certificate of Analysis
    Lot Number:
    Q3244113
    Product Catalog Number:
    07-227-I-100UG
    Product Catalog Name:
    Anti-p34-Arc/ARPC2

  • Anti-p34-Arc/ARPC2 - 3247280

    Document Type:
    Certificate of Analysis
    Lot Number:
    3247280
    Product Catalog Number:
    07-227-I-25UG
    Product Catalog Name:
    Anti-p34-Arc/ARPC2