Millipore Sigma Vibrant Logo
 

1.04419


17 Results Advanced Search  
Showing
Products (1)
Documents (15)
Site Content (1)

Narrow Your Results Use the filters below to refine your search

Document Type

  • (9)
  • (3)
  • (2)
  • (1)
Can't Find What You're Looking For?
Contact Customer Service

 

  • COA 104419

    Document Type:
    Certificate of Analysis
    Product Catalog Number:
    104419

  • A plate-based assay to measure cellular ERK substrate phosphorylation: utility for drug discovery of the MAPK-signaling cascade. 20482377

    The Ras, Raf, mitogen-activated protein kinase kinase (MEK) and extracellular signal-regulated kinase (ERK) signaling cascade is critically involved in cellular signaling with activating mutations in Ras and Raf present in many human tumors. Each constituent of this pathway is considered an important target for pharmaceutical intervention. The terminal kinase ERK is known to phosphorylate p90RSK among myriad substrates, yet robust plate-based high-throughput cellular assays monitoring such activity are not commercially available. In this study, we have utilized the Meso Scale Discovery platform to develop a plate-based assay to monitor the level of phosphorylation of p90RSK. This method is highly robust and can be used to evaluate a large number of inhibitors of ERK, MEK, or Raf in a variety of cellular backgrounds. Furthermore, this assay can be used to quantify the level of phospho-p90RSK in tumor lysates to function as a valuable pharmacodynamic readout.
    Document Type:
    Reference
    Product Catalog Number:
    04-419

  • More Than Sure

    Document Type:
    Brochure
    Product Catalog Number:
    Multiple
    Product Catalog Name:
    Multiple

  • Smart Up your lab

    Document Type:
    Brochure
    Product Catalog Number:
    Multiple
    Product Catalog Name:
    Multiple

  • Fixing

    Document Type:
    Technical Information