COA 159166
- Document Type:
- Certificate of Analysis
- Product Catalog Number:
- 159166
1.59166
8 Results
Advanced Search
Showing
Narrow Your Results Use the filters below to refine your search
Document Type
-
-
Effects of lithium and deafferentation on expression of glycogen synthase kinase-3beta, NFkappaB, beta-catenin and pCreb in the chick cochlear nucleus.
- The avian brainstem serves as a useful model to answer the question of how afferent activity influences the viability of target neurons. Approximately 20-30% of neurons in the avian cochlear nucleus, nucleus magnocellularis (NM) die following deafferentation (i.e., deafness produced by cochlea removal). Interestingly, Bcl-2 mRNA (but not protein) is upregulated in 20-30% of NM neurons following deafferentation. We have recently shown that chronic treatments of lithium upregulates the neuroprotective protein Bcl-2 and increases neuronal survival following deafferentation. The pathways leading to the upregulation of Bcl-2 expression following these two manipulations are unknown. The present experiments examine changes in glycogen synthase kinase-3 beta (Gsk-3beta), and transcription factors nuclear factor kappaB (NFkappaB), beta-catenin, and pCreb following lithium administration and following deafferentation. These molecules are known to be influenced by lithium and to regulate Bcl-2 expression in other model systems. Lithium decreased immunolabeling for Gsk-3beta and increased expression for all three transcription factors. Deafferentation, however, did not alter Gsk-3beta or NFkappaB, resulted in lower beta-catenin expression, but did increase pCreb immunoreactivity. While it is possible that pCreb is a common link in the regulation of Bcl-2 following these two manipulations, the timing and distribution of pCreb labeling suggests that it is not the sole determinant of Bcl-2 upregulation following deafferentation. It is likely that the regulation of Bcl-2 gene expression by lithium and by deafferentation involves different molecular pathways.
- Document Type:
- Reference
- Product Catalog Number:
- 06-519
- Product Catalog Name:
- Anti-phospho-CREB (Ser133) Antibody
-
Inorganics & Solvents for classical analysis
- Document Type:
- Brochure
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
-
Smart Up your lab
- Document Type:
- Brochure
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
-
More Than Sure
- Document Type:
- Brochure
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
-
Certificate of Analysis 1.120SB 5-166-001
- Document Type:
- Certificate of Analysis
- Lot Number:
- 5-166-001
- Product Catalog Number:
- 1.120SB
- Product Catalog Name:
- Readycult® Sample Bottle, Gamma Irradiated
-
Anti-Mesothelin, clone MN-1 - 3950166
- Document Type:
- Certificate of Analysis
- Lot Number:
- 3950166
- Product Catalog Number:
- MABC1140-100UG
- Product Catalog Name:
- Anti-Mesothelin Antibody, clone MN-1