NovaSeptum® Multi Sampling High Purity Application - 230411-354
- Document Type:
- Certificate of Quality
- Lot Number:
- 230411-354
- Product Catalog Number:
- 1714-10100
- Product Catalog Name:
- General sampling, high purity application, 5 x 100 mL
211354
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NOVASEPTUM® HIGH PURITY APLLICATION - 210201-354
- Document Type:
- Certificate of Quality
- Lot Number:
- 210201-354
- Product Catalog Number:
- 2711-10250
- Product Catalog Name:
- Cell Culture sampling, high purity application, 250 mL
-
c-Src is required for glial cell line-derived neurotrophic factor (GDNF) family ligand-mediated neuronal survival via a phosphatidylinositol-3 kinase (PI-3K)-dependent pa ...
- The glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs), consisting of GDNF, neurturin, persephin, and artemin, signal via a multicomponent complex composed of Ret tyrosine kinase and the glycosyl-phosphatidylinositol (GPI)-anchored coreceptors GFRalpha1-alpha4. In previous work we have demonstrated that the localization of Ret to membrane microdomains known as lipid rafts is essential for GDNF-induced downstream signaling, differentiation, and neuronal survival. Moreover, we have found that Ret interacts with members of the Src family kinases (SFK) only when it is localized to these microdomains. In the present work we show by pharmacological and genetic approaches that Src activity was necessary to elicit optimal GDNF-mediated signaling, neurite outgrowth, and survival. In particular, p60Src, but not the other ubiquitous SFKs, Fyn and Yes, was responsible for the observed effects. Moreover, Src appeared to promote neuronal survival via a phosphatidylinositol-3 kinase (PI-3K)-dependent pathway because the PI-3K inhibitor LY294002 prevented GFL-mediated neuronal survival and prevented activated Src-mediated neuronal survival. In contrast, the inhibition of Src activity had no effects on NGF-mediated survival, indicating that the requirement for Src was selective for GFL-mediated neuronal survival. These data confirm the importance of protein-protein interactions between Ret and raft-associated proteins in the signaling pathways elicited by GDNF, and the data implicate Src as one of the major signaling molecules involved in GDNF-mediated bioactivity.
- Document Type:
- Reference
- Product Catalog Number:
- 21-154
-
NOVASEPTUM® HIGH PURITY APPLICATION - 211124-351
- Document Type:
- Certificate of Quality
- Lot Number:
- 211124-351
- Product Catalog Number:
- NS711-10050
-
NOVASEPTUM® MULTI SAMPLING HIGH PURITY APPLICATION - 211124-356
- Document Type:
- Certificate of Quality
- Lot Number:
- 211124-356
- Product Catalog Number:
- 1714-10100
- Product Catalog Name:
- General sampling, high purity application, 5 x 100 mL
-
NOVASEPTUM® HIGH PURITY APPLICATION - 211124-353
- Document Type:
- Certificate of Quality
- Lot Number:
- 211124-353
- Product Catalog Number:
- 1711-10250
- Product Catalog Name:
- General sampling, high purity application, 250 mL
-
NOVASEPTUM® MULTI SAPMPLING HIGH PURITY APPLICATION - 211004-356
- Document Type:
- Certificate of Quality
- Lot Number:
- 211004-356
- Product Catalog Number:
- 2744-C0398
-
NOVASEPTUM® MULTI SAMPLING HIGH PURITY APPLICATION - 211124-355
- Document Type:
- Certificate of Quality
- Lot Number:
- 211124-355
- Product Catalog Number:
- 2714-10100
- Product Catalog Name:
- Cell Culture sampling, high purity application, 5 x 100 mL
-
NOVASEPTUM® MULTI SAMPLING HIGH PURITY APPLICATION - 221216-354
- Document Type:
- Certificate of Quality
- Lot Number:
- 221216-354
- Product Catalog Number:
- 2714-10250
- Product Catalog Name:
- Cell Culture sampling, high purity application, 5 x 250 mL
-
NOVASEPTUM® HIGH PURITY APPLICATION - 221212-354
- Document Type:
- Certificate of Quality
- Lot Number:
- 221212-354
- Product Catalog Number:
- 1711-10100
- Product Catalog Name:
- General sampling, high purity application, 100 mL