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  • NU050/452-312-AAL203256

    Document Type:
    Certificate of Quality
    Lot Number:
    AAL203256
    Product Catalog Number:
    NU050/452-312
    Product Catalog Name:
    NovAseptic Valve, Valve Body, Tank Outlet Valve 180˚, Elbow 45˚ TC

  • Anti-Luciferase - 4202334

    Document Type:
    Certificate of Analysis
    Lot Number:
    4202334
    Product Catalog Number:
    AB3256-I-25UL
    Product Catalog Name:
    Anti-Luciferase

  • Complete set of orthogonal 21st aminoacyl-tRNA synthetase-amber, ochre and opal suppressor tRNA pairs: concomitant suppression of three different termination codons in an ... 15576346

    We describe the generation of a complete set of orthogonal 21st synthetase-amber, ochre and opal suppressor tRNA pairs including the first report of a 21st synthetase-ochre suppressor tRNA pair. We show that amber, ochre and opal suppressor tRNAs, derived from Escherichia coli glutamine tRNA, suppress UAG, UAA and UGA termination codons, respectively, in a reporter mRNA in mammalian cells. Activity of each suppressor tRNA is dependent upon the expression of E.coli glutaminyl-tRNA synthetase, indicating that none of the suppressor tRNAs are aminoacylated by any of the twenty aminoacyl-tRNA synthetases in the mammalian cytoplasm. Amber, ochre and opal suppressor tRNAs with a wide range of activities in suppression (increases of up to 36, 156 and 200-fold, respectively) have been generated by introducing further mutations into the suppressor tRNA genes. The most active suppressor tRNAs have been used in combination to concomitantly suppress two or three termination codons in an mRNA. We discuss the potential use of these 21st synthetase-suppressor tRNA pairs for the site-specific incorporation of two or, possibly, even three different unnatural amino acids into proteins and for the regulated suppression of amber, ochre and opal termination codons in mammalian cells.
    Document Type:
    Reference
    Product Catalog Number:
    MAB4410

  • Anti-Luciferase, Firefly - 4292202

    Document Type:
    Certificate of Analysis
    Lot Number:
    4292202
    Product Catalog Number:
    AB3256-100UL
    Product Catalog Name:
    Anti-Luciferase Antibody, Firefly