CD14 Hu-Cy3 SmartFlare™ RNA Detection Probe
- Document Type:
- Data Sheet
cd14
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Human CD14 Monocytes / Macrophages, Fluorescein Conjugated (ready to use) - ME9CN7300
- Document Type:
- Certificate of Analysis
- Lot Number:
- ME9CN7300
- Product Catalog Number:
- MAB1219F
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CD14 Hu-Cy5 SmartFlare™ RNA Detection Probe
- Document Type:
- Data Sheet
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IL-10 enhances MD-2 and CD14 expression in monocytes and the proteins are increased and correlated in HIV-infected patients.
- Soluble proteins that bind LPS, like myeloid differentiation-2 (MD-2) and CD14, have essential roles in regulating LPS signaling through TLR4. During a gram-negative bacterial infection, the host may control the response by adjusting the levels of soluble MD-2 and CD14. To address the surface expression of MD-2 on human leukocytes, we developed a mAb, IIC1, that recognized MD-2 both free and when bound to TLR4. MD-2 was found on the surface of freshly isolated monocytes, on a subpopulation of CD19(+) B-cells and on CD15(+) neutrophils. LPS transiently reduced the MD-2 levels on monocytes, which is most likely due to endocytosis of the LPS receptor complex since MD-2 colocalized with TLR4 in early endosomes after LPS stimulation. In the absence of LPS, MD-2 partly colocalized with TLR4 in Golgi trans and medial compartments. Cultivating monocytes for 18-20 h resulted in loss of MD-2 expression on the surface, which was reversed either by LPS or IL-10. Furthermore, addition of IL-10, but not LPS, resulted in a considerable increase in mRNA for both MD-2 and CD14. Using ELISA, we demonstrated that IL-10 had a profound dose- and time-related effect on the release of soluble MD-2 and soluble CD14 from monocytes. In HIV-infected patients, the amounts of MD-2, CD14, and IL-10 increased significantly in the patient group with AIDS. Of interest, we found that IL-10, CD14, and MD-2 levels were positively correlated, suggesting that IL-10 may be a driving force for increased release of MD-2 and CD14 during systemic inflammation.
- Document Type:
- Reference
- Product Catalog Number:
- MABF117
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MOUSE ANTI-HUMAN CD14 R-PE CONJUGATED MONOCLONAL ANTIBODY - 2390422
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2390422
- Product Catalog Number:
- CBL453P
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MOUSE ANTI-HUMAN CD14 FITC CONJUGATED MONOCLONAL ANTIBODY - 2376677
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2376677
- Product Catalog Number:
- CBL453F
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MOUSE ANTI-HUMAN CD14 FITC CONJUGATED MONOCLONAL ANTIBODY - 2463636
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2463636
- Product Catalog Number:
- CBL453F
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MOUSE ANTI-HUMAN CD14 R-PE CONJUGATED MONOCLONAL ANTIBODY - 2266491
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2266491
- Product Catalog Number:
- CBL453P
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MOUSE ANTI-HUMAN CD14 FITC CONJUGATED MONOCLONAL ANTIBODY - 2111383
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2111383
- Product Catalog Number:
- CBL453F
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MOUSE ANTI-HUMAN CD14 R-PE CONJUGATED MONOCLONAL ANTIBODY -2694541
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2694541
- Product Catalog Number:
- CBL453P