- Document Type:
- Quality Management Dossier
- Product Catalog Number:
- 102092
- Product Catalog Name:
- tri-Calcium dicitrate tetrahydrate
dac
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- Document Type:
- Quality Management Dossier
- Product Catalog Number:
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- Product Catalog Number:
- 104005
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Anti-His Tag, clone 4D11, Alexa Fluor® 488 conjugate (mouse monoclonal IgG2aκ) - 2398836
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2398836
- Product Catalog Number:
- 16-254
- Product Catalog Name:
- Anti-Histidine Tagged Antibody, clone 4D11, Alexa Fluor® 488 conjugate
-
G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity.
- The recognition specificity of monoclonal antibodies (mAbs) has made mAbs among the most frequently used tools in both basic science research and in clinical diagnosis and therapies. Precise determination of the epitope allows the development of epitope tag systems to be used with recombinant proteins for various purposes. Here we describe a new family of tag derived from the epitope recognized by a highly specific mAb G196. The minimal epitope was identified as the five amino acid sequence Asp-Leu-Val-Pro-Arg. Permutation analysis was used to characterize the binding requirements of mAb G196, and the variable regions of the mAb G196 were identified and structurally analyzed by X-ray crystallography. Isothermal titration calorimetry revealed the high affinity (Kd = 1.25 nM) of the mAb G196/G196-epitope peptide interaction, and G196-tag was used to detect several recombinant cytosolic and nuclear proteins in human and yeast cells. mAb G196 is valuable for developing a new peptide tagging system for cell biology and biochemistry research.
- Document Type:
- Reference
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
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Anti-His Tag, clone 4D11, Alexa Fluor#174; 488 conjugate (mouse monoclonal IgG2a#954;) - 2142156
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2142156
- Product Catalog Number:
- 16-254
- Product Catalog Name:
- Anti-Histidine Tagged Antibody, clone 4D11, Alexa Fluor® 488 conjugate
-
Anti-Myc Tag, clone 9E10, Alexa Fluor® 488 conjugate (mouse monoclonal IgG1) - 2377353
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2377353
- Product Catalog Number:
- 16-308
- Product Catalog Name:
- Anti-Myc Tag Antibody, clone 9E10, Alexa Fluor® 488 conjugate
-
Anti-His Tag, clone 4D11, Alexa Fluor#174; 488 conjugate (mouse monoclonal IgG2a#954;) - DAM1831489
- Document Type:
- Certificate of Analysis
- Lot Number:
- DAM1831489
- Product Catalog Number:
- 16-254
- Product Catalog Name:
- Anti-Histidine Tagged Antibody, clone 4D11, Alexa Fluor® 488 conjugate
-
Anti-His Tag, clone 4D11, Alexa Fluor® 555 conjugate (mouse monoclonal IgG2aκ) - 2344704
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2344704
- Product Catalog Number:
- 16-255
-
Anti-His Tag, clone 4D11, Alexa Fluor® 488 conjugate (mouse monoclonal IgG2a) - 2299518
- Document Type:
- Certificate of Analysis
- Lot Number:
- 2299518
- Product Catalog Number:
- 16-254
- Product Catalog Name:
- Anti-Histidine Tagged Antibody, clone 4D11, Alexa Fluor® 488 conjugate